c-Src kinase domain Thr338Ile mutant in complex with ATPgS

PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER
ATP-GAMMA-S; ADENOSINE 5'-(3-THIOTRIPHOSPHATE); ADENOSINE 5'-(GAMMA-THIOTRIPHOSPHATE); ADENOSINE-5'-DIPHOSPHATE MONOTHIOPHOSPHATE

Activation of tyrosine kinases by mutation of the gatekeeper threonine.

Proto-oncogene tyrosine-protein kinase Src

Coexpressed with YopH phosphatase and GroEL/Trigger Factor. (Seeliger et al Protein Science, 2005 Dec;14(12):3135-9)

10% PEG 4000, 50 mM Ammonium acetate, 100 mM Bis-Tris pH 5.5, 5% Glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 293K

SRC, KINASE, ACTIVE, GATEKEEPER, ATP-binding, Lipoprotein, Myristate, Nucleotide-binding, Phosphoprotein, Proto-oncogene, SH2 domain, SH3 domain, Transferase, Tyrosine-protein kinase