Crystal structure of the Asn-bound guinea pig L-asparaginase 1 catalytic domain active site mutant K188M

Experimental Data in Support of a Direct Displacement Mechanism for Type I/II l-Asparaginases.

The first 23 residues are from the tag hexahistidine tag and TEV protease cleavage site. They were not removed during purification but are not seen in the structure. The C-terminus of the protein was cleaved in the drop and is not seen in the structure despite being present throughout the entirety of the purification.

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS