Fitting of the eEF2 crystal structure into the cryo-EM density map of the eEF2.80S.AlF4-.GDP complex

Visualization of the eEF2-80S ribosome transition-state complex by cryo-electron microscopy.

METHOD--Each domain fitted as rigid body, domains I (G and G), II,IV, and V were fitted. Domain III, and domain IV insertions were not included in this fitting model. The coordinates for this entry are based on manual fitting of the coordinates of the crystal structure 1N0U into cryo-EM density map. Therefore, authors did not deposit new structure factors.

supervised classification was used (ref. Valle, M. et al, 2002, EMBO J.)

20 mM Hepes-NH3, 100 mM KCl, 20 mM MgCl2

segregation in defocus groups and correction in volumes

eEF2-bound 80S complex in presence of AlF4-, and GDP

EF-2, Translation elongation factor 2, Eukaryotic elongation factor 2, eEF2, Ribosomal translocase

Crystal structure of yeast elongation factor 2 in complex with sordarin

Cryo-EM map for eEF2.80S.AlF4-.GDP complex

Isolated density of the eEF2, segmented out from cryo-EM map for eEF2.80S.AlF4-.GDP complex (from EMD-5015)

eEF2 transition state complex, 80S Ribosome, AlF4-, GDP, GTPase, Translocation, Elongation factor, GTP-binding, Nucleotide-binding, Phosphoprotein, Protein biosynthesis, RNA-binding, rRNA-binding, CELL CYCLE