Molecular structure for the HIV-1 gp120 trimer in the CD4-bound state

Molecular architecture of native HIV-1 gp120 trimers.

METHOD--Automatic. The coordinates for this entry and the two related entries 3DNL and 3DNN are based on fitting 1GC1 coordinates for the gp120 monomer deposited previously by Kwong et al in 1998 into the density map for the trimer derived by electron microscopy. Therefore, authors did not deposit new structure factors, and, any features such as unusual torsion angles are the same as in the 1GC1 coordinates. REFINEMENT PROTOCOL--rigid body

IMOD was used for tomographic reconstruction. Programs developed in-house were used for alignment and classification of individual tomographic volumes. The final map is the average of ~4000 individual spikes.
Tomographic reconstruction Weighted Back Projection

EM MAP for Native HIV-1 envelope glycoprotein in the CD4-bound state (this conformation).

EM MAP for Native HIV-1 envelope glycoprotein in the b12-bound state.

EM MAP for Native HIV-1 envelope glycoprotein in the unliganded state.

HIV-1 GP120 CORE COMPLEXED WITH CD4 AND A NEUTRALIZING HUMAN ANTIBODY

Molecular structure for the HIV-1 gp120 trimer in the b12-bound state.

Molecular structure for the HIV-1 gp120 trimer in the unliganded state.

HIV-1, ENVELOPE GLYCOPROTEIN, IMMUNODEFICIENCY VIRUS, gp120, AIDS, Apoptosis, Cleavage on pair of basic residues, Envelope protein, Fusion protein, Host-virus interaction, Lipoprotein, Membrane, Palmitate, Viral immunoevasion, Virion, VIRAL PROTEIN