HUMAN MYELOPEROXIDASE-CYANIDE-THIOCYANATE COMPLEX

Human myeloperoxidase: structure of a cyanide complex and its interaction with bromide and thiocyanate substrates at 1.9 A resolution.

MYELOPEROXIDASE LIGHT CHAIN CONTAINING RESIDUES 1 TO 104

MYELOPEROXIDASE HEAVY CHAIN CONTAINING RESIDUES 113 TO 578

Vapor Diffusion, Hanging Drop with Macro Seeding
Co-crystal grown from sodium cyanide, sodium acetate, ammonium, sulfate, calcium chloride, and PEG MW 8000. It was subsequently equilibrated with a substitute mother liquor containing sodium thiocyanate, pH 5.5, Vapor Diffusion, Hanging Drop with Macro Seeding, temperature 22K

Crystal Structure of Bromide-bound Human Myeloperoxidase Isoform C at pH 5.5

1MHL contains the native protein structure solved to 2.25 Angstroms resolution

1MYP contains the canine myeloperoxidase structure at 3.0 Angstroms resolution

CRYSTAL STRUCTURE OF CYANIDE-BOUND HUMAN MYELOPEROXIDASE ISOFORM C AT PH 5.5

1D7W contains the 1.9 Angstrom resolution structure of human myeloperoxidase complexed with cyanide and bromide

Structure of Native Myeloperoxidase at 1.8 angstroms

Sturctural Analysis of Human Myeloperoxidase-Thiocyanate Complex

Slow Cooling Method with
Simulated Annealing and Bulk
Solvent Correction

Random selection of 5% of
observed reflections

MYELOPEROXIDASE (E.C.1.11.1.7)/CYANIDE-THIOCYANATE COMPLEX

The biological assembly is a homodimer
formed from two pseudo-monomers made
from a light and a heavy chain.
The monomer's symmetry partner
is generated by rotation about
a noncrystallographic two-fold.

oxidoreductase, peroxidase, substrate complex, thiocyanate, halide peroxidation