CryoEM structure of Pseudomonas aeruginosa PA14 JetC ATPase domain bound to DNA and cWHD domain of JetA

PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER
ATP-GAMMA-S; ADENOSINE 5'-(3-THIOTRIPHOSPHATE); ADENOSINE 5'-(GAMMA-THIOTRIPHOSPHATE); ADENOSINE-5'-DIPHOSPHATE MONOTHIOPHOSPHATE

The SMC-family Wadjet complex protects bacteria from plasmid transformation by recognition and cleavage of closed-circular DNA.

Prepared using deionized water and filtered sterilized.

JetC head ATPase domain in complex with DNA, and cWHD of JetA

cWHD domain of JetA observed in complex with dimeric JetC.

Below mentioned hybridized oligonucleotides were used in the sample preparation. Considering the DNA binding is purely based on the electrostatic charge: polyA/polyT chain was used to generate the model. JetABC_cryo_Fwd: GTGATAGTTAGAAACGTAATTGACTATAAAGATGATGACGATAAGTAGGATGTCTATAGACCAGG JetABC_cryo_Rev: CCTGGTCTATAGACATCCTACTTATCGTCATCATCTTTATAGTCAATTACGTTTCTAACTATCAC

National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)

The head domains of JetC interacts with DNA in presence of ATP or ATP analog.

Wadjet, Bacterial defense systems, JetA, JetC, Anti-plasmid defense system, EptA, EptC, MksB, MksF, SMC, DNA BINDING PROTEIN-DNA complex