A conformationally strained, circular permutant of barnase

Structural and thermodynamic analysis of a conformationally strained circular permutant of barnase.

1M Tris, 14% peg 8k, 150mM (NH4)2SO4, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K

CIRCULAR PERMUTANT, PROTEIN-PROTEIN COMPLEX, ENDONUCLEASE, Cytoplasm, PROTEIN BINDING