Cryo-EM structures of ribosomal 80S complexes with termination factors and cricket paralysis virus IRES reveal the IRES in the translocated state

Cryo-Em of Ribosomal 80S Complexes with Termination Factors Reveals the Translocated Cricket Paralysis Virus Ires.

CROSS- -CORRELATION DENSITIES WITH REFERENCE STRUCTURE

20 MM TRIS PH 7.5, 100 MM KCL, 1 MM DTT, 8.5 MM MGCL2, 0.133 MM GTP, 2.33 MM GMPPNP

MICROGRAPHS WERE SELECTED FOR DRIFT AND ASTIGMATISM
CRICKET PARALYSIS VIRUS IRES RNA BOUND TO MAMMALIAN 80S RIBOSOME, ERF1 AND ERF3

GUANINE NUCLEOTIDE-BINDING PROTEIN SUBUNIT BETA-2-LIKE 1

EUKARYOTIC PEPTIDE CHAIN RELEASE FACTOR SUBUNIT 1

EUKARYOTIC PEPTIDE CHAIN RELEASE FACTOR GTP-BINDING SUBUNIT ERF3A

40S RIBOSOMAL PROTEIN US2,37 KDA LAMININ RECEPTOR PRECURSOR, 37LRP, 37/67 KDA LAMININ RECEPTOR, LRP/LR, 67 KDA LAMININ RECEPTOR, 67LR, COLON CARCINOMA LAMININ-BINDING PROTEIN, LAMININ RECEPTOR 1, LAMR, LAMININ-BINDING PROTEIN PRECURSOR P40, LBP/P40, MULTIDRUG RESISTANCE-ASSOCIATED PROTEIN MGR1-AG, NEM/1CHD4

40S RIBOSOMAL PROTEIN ES1, V-FOS TRANSFORMATION EFFECTOR PROTEIN, FTE-1

40S RIBOSOMAL PROTEIN US5, PROTEIN LLREP3

40S RIBOSOMAL PROTEIN ES6, PHOSPHOPROTEIN NP33

40S RIBOSOMAL PROTEIN ES27, METALLOPAN-STIMULIN 1, MPS-1

40S RIBOSOMAL PROTEIN ES31, UBIQUITIN CARBOXYL EXTENSION PROTEIN 80

CELL PROLIFERATION-INDUCING GENE 21 PROTEIN, GUANINE NUCLEO TIDE-BINDING PROTEIN SUBUNIT BETA-LIKE PROTEIN 12.3, HUMAN LUNG CA NCER ONCOGENE 7 PROTEIN, HLC-7, RECEPTOR FOR ACTIVATED C KINASE, R ECEPTOR OF ACTIVATED PROTEIN KINASE C 1, RACK1

EUKARYOTIC RELEASE FACTOR 1, ERF1, PROTEIN CL1, TB3-1,

EUKARYOTIC PEPTIDE CHAIN RELEASE FACTOR SUBUNIT 3A, ERF3A, G1 TO S PHASE TRANSITION PROTEIN 1 HOMOLOG

SHEET
DETERMINATION METHOD: DSSP
THE SHEETS PRESENTED AS "BB" IN EACH CHAIN ON SHEET RECORDS
BELOW IS ACTUALLY AN -7-STRANDED BARREL THIS IS REPRESENTED BY
A -6-STRANDED SHEET IN WHICH THE FIRST AND LAST STRANDS
ARE IDENTICAL.

THE SHEETS PRESENTED AS "BD" IN EACH CHAIN ON SHEET RECORDS
BELOW IS ACTUALLY AN -2-STRANDED BARREL THIS IS REPRESENTED BY
A -1-STRANDED SHEET IN WHICH THE FIRST AND LAST STRANDS
ARE IDENTICAL.

40S RIBOSOMAL PROTEIN SA, 40S RIBOSOMAL PROTEIN S3A, 40S RIBOSOMAL PROTEIN S2, 40S RIBOSOMAL PROTEIN S4, Y ISOFORM 1, 40S RIBOSOMAL PROTEIN S5, 40S RIBOSOMAL PROTEIN S6, 40S RIBOSOMAL PROTEIN S7, 40S RIBOSOMAL PROTEIN S8, 40S RIBOSOMAL PROTEIN S9, 40S RIBOSOMAL PROTEIN S10, 40S RIBOSOMAL PROTEIN S11, 40S RIBOSOMAL PROTEIN S12, 40S RIBOSOMAL PROTEIN S13, 40S RIBOSOMAL PROTEIN S14, 40S RIBOSOMAL PROTEIN S15, 40S RIBOSOMAL PROTEIN S16, 40S RIBOSOMAL PROTEIN S17, 40S RIBOSOMAL PROTEIN S18, 40S RIBOSOMAL PROTEIN S19, 40S RIBOSOMAL PROTEIN S20, 40S RIBOSOMAL PROTEIN S21, 40S RIBOSOMAL PROTEIN S15A, 40S RIBOSOMAL PROTEIN S23, 40S RIBOSOMAL PROTEIN S24, 40S RIBOSOMAL PROTEIN S25, 40S RIBOSOMAL PROTEIN S26, 40S RIBOSOMAL PROTEIN S27, 40S RIBOSOMAL PROTEIN S28, 40S RIBOSOMAL PROTEIN S29, 40S RIBOSOMAL PROTEIN S30, UBIQUITIN-40S RIBOSOMAL PROTEIN S27A, GUANINE NUCLEOTIDE-BINDING PROTEIN SUBUNIT BETA-2-LIKE 1, EUKARYOTIC PEPTIDE CHAIN RELEASE FACTOR SUBUNIT 1, EUKARYOTIC PEPTIDE CHAIN RELEASE FACTOR GTP-BINDING SUBUNIT ERF3A/RNA Complex