HcgB from Methanocaldococcus jannaschii with the pyridinol derived from FeGP cofactor of [Fe]-hydrogenase

(4,6-dihydroxy-3,5-dimethylpyridin-2-yl)acetic acid

Towards artificial methanogenesis: biosynthesis of the [Fe]-hydrogenase cofactor and characterization of the semi-synthetic hydrogenase.

Uncharacterized protein MJ0488, Guanylyltransferase

0.1 M sodium cacodylate (pH 6.5), 20 % (w/v) PEG8000, 0.2 M magnesium acetate