Cryo-EM structure of Schizosaccharomyces pombe Atg9

Atg9 is a lipid scramblase that mediates autophagosomal membrane expansion.

N-(2-Hydroxyethyl)piperazine-N'-2-ethanesulfonic Acid

Protein detergent complex (Atg9-LMNG ) treated with GraFix

Schizosaccharomyces pombe (strain 972 / ATCC 24843)

The grid was washed by acetone prior to use

A point mutation, S687Y, in the unobserved regions, is an unexpected mutation during plasmid preparation. C-terminal residues is linker (Gly-Ser) and cleaved site (Leu-Glu-Val-Leu-Phe-Gln).

Japan Society for the Promotion of Science (JSPS)

Japan Agency for Medical Research and Development (AMED)

Autophagy, membrane protein, UNKNOWN FUNCTION