Crystal structure of the RNA-helicase Prp43 from Chaetomium thermophilum bound to ADP

Structural and functional analysis of the RNA helicase Prp43 from the thermophilic eukaryote Chaetomium thermophilum.

The protein was expressed with 2 additional N-terminal amino acids (MA) and a C-terminal Strep-tag (WSHPQFEK).
Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP43

Chaetomium thermophilum var. thermophilum DSM 1495

8 % (w/v) Jeffamine M2070, 0.17 M Glycine, 16.7 % (v/v) DMSO, 10 mM Urea

Spliceosome, RNA-helicase, DEAH-box protein, DHX15, hydrolase