Deep classification of a large cryo-EM dataset defines the conformational landscape of the 26S proteasome

SUBMISSION BASED ON EXPERIMENTAL DATA FROM EMDB EMD-2594. (DEPOSITION ID: 12355).

VITRIFICATION 1 -- CRYOGEN- ETHANE, INSTRUMENT- HOMEMADE PLUNGER,

26S PROTEASE REGULATORY SUBUNIT 7 HOMOLOG

26S PROTEASE REGULATORY SUBUNIT 4 HOMOLOG

26S PROTEASE REGULATORY SUBUNIT 8 HOMOLOG

26S PROTEASE REGULATORY SUBUNIT 6B HOMOLOG

20S PROTEASOME BETA SUBUNIT 1, MACROPAIN SUBUNIT PRE3, MULTICATALYTIC ENDOPEPTIDASE COMPLEX SUBUNIT PRE3, PROTEINASE YSCE SUBUNIT PRE3

20S PROTEASOME BETA SUBUNIT 2 MACROPAIN SUBUNIT PUP1, MULTICATALYTIC ENDOPEPTIDASE COMPLEX SUBUNIT PUP1, PROTEINASE YSCE SUBUNIT PUP1

20S PROTEASOME BETA SUBUNIT 3, MACROPAIN SUBUNIT PUP3, MULTICATALYTIC ENDOPEPTIDASE COMPLEX SUBUNIT PUP3

20S PROTEASOME BETA SUBUNIT 4, MACROPAIN SUBUNIT C11, MULTICATALYTIC ENDOPEPTIDASE COMPLEX SUBUNIT C11, PROTEINASE YSCE SUBUNIT 11

20S PROTEASOME BETA SUBUNIT 5, MACROPAIN SUBUNIT PRE2, MULTICATALYTIC ENDOPEPTIDASE COMPLEX SUBUNIT PRE2, PROTEINASE YSCE SUBUNIT PRE2

20S PROTEASOME BETA SUBUNIT 6, MULTICATALYTIC ENDOPEPTIDASE

20S PROTEASOME BETA SUBUNIT 7, MACROPAIN SUBUNIT PRE4, MULTICATALYTIC ENDOPEPTIDASE COMPLEX SUBUNIT PRE4, PROTEINASE YSCE SUBUNIT PRE4

20S PROTEASOME ALPHA SUBUNIT 1, MACROPAIN SUBUNIT C7-ALPHA, MULTICATALYTIC ENDOPEPTIDASE COMPLEX C7, PROTEASOME COMPONENT Y8, PROTEINASE YSCE SUBUNIT 7, SCL1 SUPPRESSOR PROTEIN

20S PROTEASOME ALPHA SUBUNIT 2, MACROPAIN SUBUNIT Y7, MULTICATALYTIC ENDOPEPTIDASE COMPLEX SUBUNIT Y7, PROTEINASE YSCE SUBUNIT 7

20S PROTEASOME ALPHA SUBUNIT 3, MACROPAIN SUBUNIT Y13, MULTICATALYTIC ENDOPEPTIDASE COMPLEX SUBUNIT Y13, PROTEINASE YSCE SUBUNIT 13

20S PROTEASOME ALPHA SUBUNIT 4, MACROPAIN SUBUNIT PRE6, MULTICATALYTIC ENDOPEPTIDASE COMPLEX SUBUNIT PRE6, PROTEINASE YSCE SUBUNIT PRE6

20S PROTEASOME ALPHA SUBUNIT 5, MACROPAIN SUBUNIT PUP2, MULTICATALYTIC ENDOPEPTIDASE COMPLEX SUBUNIT PUP2, PROTEINASE YSCE SUBUNIT PUP2

20S PROTEASOME ALPHA SUBUNIT 6, MACROPAIN SUBUNIT PRE5, MULTICATALYTIC ENDOPEPTIDASE COMPLEX SUBUNIT PRE5, PROTEINASE YSCE SUBUNIT PRE5

20S PROTEASOME ALPHA SUBUNIT 7, MACROPAIN SUBUNIT C1, MULTICATALYTIC ENDOPEPTIDASE COMPLEX SUBUNIT C1, PROTEINASE YSCE SUBUNIT 1

RPT1, PROTEIN CIM5, TAT-BINDING HOMOLOG 3

RPT6, PROTEIN CIM3, PROTEIN SUG1, TAT-BINDING PROTEIN TBY1

RPT3, PROTEIN YNT1, TAT-BINDING HOMOLOG 2

RPT4,26S PROTEASE SUBUNIT SUG2, PROTEASOMAL CAP SUBUNIT

RPT5, TAT-BINDING PROTEIN HOMOLOG 1, TBP-1

HMG-COA REDUCTASE DEGRADATION PROTEIN 2, PROTEASOME NON-ATPASE SUBUNIT 1

SHEET
THE SHEET STRUCTURE OF THIS MOLECULE IS BIFURCATED. IN
ORDER TO REPRESENT THIS FEATURE IN THE SHEET RECORDS BELOW,
TWO SHEETS ARE DEFINED.

LYS 108 ARG CONFLICT IN CHAIN 5 IS DUE TO A MUTATION IN THE STARTING MODEL PDB ENTRY 1RYP