Crystal structure of the E.coli LeuRS-tRNA complex with the non- cognate isoleucyl adenylate analogue

The Physiological Target for Leurs Translational Quality Control is Norvaline

ESCHERICHIA COLI TRNA-LEU UAA ISOACCEPTOR

LEUCYL-TRNA SYNTHETASE, LEURS, LEUCY-TRNA SYNTHETASE

0.1 M BIS-TRIS (PH 5.5), 23-25 % PEG 3350, 0-200 MM AMMONIUM ACETATE

CONTAINS A N-TERM HIS-TAG MGSSHHHHHHSSGLVPRGSH

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS. HYDROGENS HAVE BEEN USED IF PRESENT IN THE INPUT. U VALUES REFINED INDIVIDUALLY

LIGASE-RNA COMPLEX, LIGASE, TRANSLATIONAL FIDELITY, ADAPTIVE RESPONSE AND EVOLUTION