Crystal structure of human NQO1 by serial femtosecond crystallography

Modular droplet injector for sample conservation providing new structural insight for the conformational heterogeneity in the disease-associated NQO1 enzyme.

Azoreductase,DT-diaphorase,DTD,Menadione reductase,NAD(P)H:quinone oxidoreductase 1,Phylloquinone reductase,Quinone reductase 1,QR1

0.1 M Tris pH 8.5, 0.2 M sodium acetate, 20% polyethylene glycol (PEG) 3350, 20 um FAD

Spanish Ministry of Science, Innovation, and Universities

Crystals in mother liquor encapsulated into oil droplets

Hydrogens have been added in their riding positions

NQO1, flavoprotein, cancer, serial femtosecond crystallography, microcrystals, XFEL, Droplet injection, OXIDOREDUCTASE

Global NCS restraints between domains: 1 2

Global NCS restraints between domains: 3 4

Global NCS restraints between domains: 5 6

Global NCS restraints between domains: 7 8

Global NCS restraints between domains: 9 10

Global NCS restraints between domains: 11 12