Crystal structure of Trypanosoma cruzi CYP51 bound to the inhibitor (R)-N-(3-(1H-indol-3-yl)-1-oxo-1-(pyridin-4-ylamino)propan-2-yl)-3,3'- difluoro-(1,1'-biphenyl)-4-carboxamide

R-Configuration of 4-Aminopyridyl-Based Inhibitors of Cyp51 Confers Superior Efficacy Against Trypanosoma Cruzi

32 N-TERMINUS RESIDUES ARE REPLACED WITH THE SEQUENCE MAKKTSSKGKL, 6XHIS TAG ENGINEERED AT THE C- TERMINUS

TC14DM, CYTOCHROME P450 51, LANOSTEROL 14-ALPHA DEMETHYLASE

0.2 M AMMONIUM SULFATE; 0.1 M BIS-TRIS, PH 6.5; 25% PEG 3550

PROTOPORPHYRIN IX CONTAINING FE (HEM): THIOLATE BOND TO CYS 422
FIRST 32 RESIDUES AT THE N-TERMINUS ARE REPLACED WITH THE MAKKTSSKGKL SEQUENCE, 6XHIS TAG ENGINEERED AT THE C- TERMINUS

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS.

STEROL 14-ALPHA DEMETHYLASE (E.C.1.14.13.70)

OXIDOREDUCTASE, STEROL 14-DEMETHYLASE, STEROL BIOSYNTHESIS, CHAGAS DISEASE