A complex between a variant of staphylococcal enterotoxin C3 and the variable domain of the murine T cell receptor beta chain 8.2

Manipulating the coupled folding and binding process drives affinity maturation in a protein-protein complex

20% PEG 3350, 0.2 M tri-ammonium citrate pH 7.0, 0.3 % dioxane, VAPOR DIFFUSION, HANGING DROP, temperature 298K

RESIDUES 128-133 (VGKVTG) OF UNP ENTRY P0A0L5 WERE REPLACED WITH RESIDUES 100-104 (KASTWH) IN CHAINS B,D,F,AND H OF THE CRYSTALLIZED SEQUENCE.

Murine T cell receptor beta chain 8.2, Enterotoxin type C-3