Negative stain EM reconstruction of M.tuberculosis Acr1(Hsp 16.3) fitted with wheat sHSP dimer

Dodecameric Structure of the Small Heat Shock Protein Acr1 from Mycobacterium Tuberculosis.

METHOD--QUALITY OF THE FIT R-FACTOR= 0.516, CROSS- CORRELATION COEFFICIENT 81.5%

DIMER ALPHA CRYSTALLIN DOMAIN OF 1GME (43-137 AND 146-151) FITTED INTO DENSITY USING URO. RESOLUTION WAS DETERMINED BY 0.5 FOURIER SHELL CORRELATION.

THIS ENTRY CONTAINS THE COORDINATES OF WHEAT HSP 16.9 WHICH HAVE BEEN FITTED INTO THE CRYO-EM RECONSTRUCTION OF HSP 16.3 FROM M.TUBERCULOSIS. THE COMPND AND SOURCE RECORDS GIVE DETAILS OF THE PROTEIN REPRESENTED BY THE COORDINATES, RATHER THAN THE PROTEIN FROM WHICH THE EM MAP WAS DERIVED

THE PROTEIN MODEL WAS OBTAINED FROM PDB ENTRY 1GME

CRYSTAL STRUCTURE AND ASSEMBLY OF AN EUKARYOTIC SMALL HEAT SHOCK PROTEIN

COOORDINATES DERIVED FROM WHEAT SHSP PDB 1GME THEREFORE PDB SEQUENCE IS NOT THAT OF THE PROTEIN

SMALL HEAT SHOCK PROTEIN, ALPHA-CRYSTALLIN, CHAPERONE