Double-motif Single-stranded Paranemic Crossover RNA Triangle (2PXT)

An RNA Paranemic Crossover Triangle as A 3D Module for Cotranscriptional Nanoassembly.

Patch CTF estimation (multi) from cryoSPARC.

In vitro transcribed RNA purified by SEC.

Sample was purified by size exclusion chromatography and concentrated in an Amicon spin concentrator.

3 microliter droplet, 4 second delay before blotting, 6 second blot, 0 second delay before plunging.

RNA origami, Nanostructure, self-assembly, RNA