Mn2+Directly Activates cGAS and Structural Analysis Suggests Mn2+Induces a Noncanonical Catalytic Synthesis of 2'3'-cGAMP.

m-cGAS,2'3'-cGAMP synthase,Mab-21 domain-containing protein 1

0.2 M Ammonium sulfate, 0.1 M MES, 30 % w/v PEG-5000 MME, pH 6.5

National Science Foundation (NSF, China)

cGAS, activator, inverted-orientation, metal-ion binding protein, IMMUNE SYSTEM

binding site for Di-nucleotide A A 602 and 5GP A 603

binding site for Di-nucleotide A B 602 and 5GP B 603