T1 lipase mutant - 5M (D43E/T118N/E226D/E250L/N304E)

Structure elucidation and docking analysis of 5M mutant of T1 lipase Geobacillus zalihae.

0.5 M sodium cacodylate trihydrate, 0.4 M sodium citrate tribasic pH 6.5, 0.2 M NaCl

This is D43E/T118N/E226D/E250L/N304E mutant. And 18 N-terminal residues (MSPISRHHHHHHLVPRGS) are expression tags.

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS U VALUES : REFINED INDIVIDUALLY

lipase, mutation, triglycerides, HYDROLASE