Golgi mannosidase II D204A catalytic nucleophile mutant with an empty active site

N-acetyl-beta-D-glucosamine; 2-acetamido-2-deoxy-beta-D-glucose; 2-acetamido-2-deoxy-D-glucose; 2-acetamido-2-deoxy-glucose; N-ACETYL-D-GLUCOSAMINE

Probing the substrate specificity of Golgi alpha-mannosidase II by use of synthetic oligosaccharides and a catalytic nucleophile mutant.

Alpha-mannosidase II, Mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase, MAN II, Golgi alpha-mannosidase II, AMAN II

Tris, NaCl, PEG6000, MPD;
Tris found in the active site under normal
crystallization conditions was removed by
soaking crystals in phosphate buffered
resevoir solution, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K

dGMII + 5-thio-D-mannopyranosylamidinium salt

dGMII + (2R,3R,4S)-2-({[(1R)-2-hydroxy-1-phenylethyl]amino}methyl)pyrrolidine-3,4-diol

dGMII + (2R,3R,4S)-2-({[(1S)-2-hydroxy-1-phenylethyl]amino}methyl)pyrrolidine-3,4-diol

dGMII + (2R,3R,4S,5R)-2-({[(1R)-2-hydroxy-1-phenylethyl]amino}methyl)-5-methylpyrrolidine-3,4-diol

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS

GLYCOSYL HYDROLASE FAMILY 38, Glycosidase, Golgi apparatus, Membrane, Signal-anchor, Transmembrane, HYDROLASE