Switching GFP fluorescence using genetically encoded phenyl azide chemistry through two different non-native post-translational modifications routes at the same position.

{2-[(1R,2R)-1-amino-2-hydroxypropyl]-4-(4-hydroxybenzylidene)-5-oxo-4,5-dihydro-1H-imidazol-1-yl}acetic acid

Molecular basis for functional switching of GFP by two disparate non-native post-translational modifications of a phenyl azide reaction handle.

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS U VALUES : WITH TLS ADDED

synthetic biology, photocontrol, optogenetics, unnatural amino acids, protein fluorescence, sfGFP, fluorescent protein