Dehydratase domain (DH) of a mycocerosic acid synthase-like (MAS-like) PKS, crystal form 2

Mycocerosic acid synthase exemplifies the architecture of reducing polyketide synthases.

Mycocerosic acid synthase-like polyketide synthase

Escherichia coli 'BL21-Gold(DE3)pLysS AG'

Crystallization condition:
16 % (w/v) PEG 3350
0.25 M disodium malonate
0.1 M BIS-TRIS propane pH 6.5
Protein/buffer:
38 mg/ml protein
0.02 M HEPES pH 7.4
0.25 M NaCl
5 % (v/v) Glycerol
0.005 M DTT
Cryo:
25 % (v/v) Ethylene glycol (final)

Mycocerosic acid synthase (E.C.2.3.1.111)