Ribosome binding of a single copy of the SecY complex: implications for protein translocation

Chirality error at GLN241, GLN250, and ALA373 in chain A.

REFINEMENT PROTOCOL--rigid body, followed by manual rebuilding and extension of the loops

50 mM HEPES- KOH pH 7.5, 100 mM KOAc, 10 mM Mg(OAc)2, 0.05% DDM

EMAN- phase flipping of particles form the same micrograph

Solid carbon on a holey film, 400 mesh Cu grid

The specimens were plunge frozen in liquid ethane at 4 degrees C at an RH of ~90-95%.

PROTEIN TRANSPORT PROTEIN SEC61 GAMMA SUBUNIT

Protein transport protein SEC61 subunit beta homolog

THIS PDB FILE WAS OBTAINED UPON RIGID BODY REFINEMENT OF A STARTING PDB FILE INTO A CRYO-EM MAP. THE E. COLI RIBOSOMES ARE OBTAINED NATURALLY, BUT FOR THE MODELING OF THE PROTEINS INTO THE EM MAP, AUTHORS SOMETIMES DID NOT MODEL IN THE ENTIRE PROTEIN.

Ribosome-SecY complex, protein translocation, RIBOSOME