Myosin VI nucleotide-free (MDinsert2-IQ) crystal structure

The Structure of the Myosin Vi Motor Reveals the Mechanism of Directionality Reversal

8-10% PEG 8000, 50MM MES PH 6.7, 150MM NH4.SO4, 3% ISO-PROPANOL, 3% TERT-BUTANOL

MYOSIN VI NUCLEOTIDE-FREE (MDINSERT2) CRYSTAL STRUCTURE

CALMODULIN MUTANT WITH A TWO RESIDUE DELETION IN THE CENTRAL HELIX

THE AUTHORS STATE THAT THE ORIGINAL SEQUENCE (UNIPROT Q29122) OF MYOSIN VI FROM PIG WAS MOST LIKELY INCORRECT BECAUSE THE CHANGES THAT ARE IN THEIR CLONE (LYS DELETION AND THE 6 MUTATIONS) ARE CONSERVED ACROSS THE MYOSIN VI FAMILY.

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS. CAM BOUND TO THE IQ MOTIF IS POORLY DEFINED IN THE DENSITY, ONLY POLY-ALA CHAINS HAVE BEEN MODELED FOR IT.

MOTOR PROTEIN-METAL-BINDING PROTEIN COMPLEX, COMPLEX (MOTOR PROTEIN-CALMODULIN), MYOSIN VI, REVERSE MYOSIN, CALMODULIN, IQ MOTIF, NON- CONVENTIONAL MYOSIN, NUCLEOTIDE-FREE CONFORMATION, MUSCLE PROTEIN