A de novo designed nonameric coiled coil, CC-Type2-(GgLaId)4

Coiled coils 9-to-5: rational de novo design of alpha-helical barrels with tunable oligomeric states.

Nonameric de novo coiled coil CC-Type2-(GgLaId)4

0.1 M ammonium acetate, 0.05 M Na HEPES, 12.5% v/v propan-2-ol. 25% v/v glycerol added to crystal as cryo-protectant prior to cryo-cooling.

Biotechnology and Biological Sciences Research Council (BBSRC)

Nonameric open barrel has only been observed in a crystalline environment. Same sequence also crystallises as a collapsed hexameric barrel, furthermore AUC (both SV and SE) experiments indicate that that the sequence is hexameric in aqueous solution.

Repeated attempts to model solvent within the observed density in the centre of each barrel pore were inconsistent. Therefore we have included representative solvent molecules to fit the density as well as possible; however we note that the fit is poor, and that these solvent molecules do not make the expected stabilising contacts with the surrounding protein.

coiled coil, alpha-helical barrel, nonamer, synthetic, DE NOVO PROTEIN