Crystal structure of ubiquitin charged Ube2N (Ube2N~Ub) in complex with Ube2V2

2-Gaussian fit: Grosse-Kunstleve RW, Sauter NK, Adams PD: Newsletter of the IUCr Commission on Crystallographic Computing 2004, 3, 22-31.

RING domains act as both substrate and enzyme in a catalytic arrangement to drive self-anchored ubiquitination.

Ubiquitin-conjugating enzyme E2 variant 2

DDVit 1,Enterocyte differentiation-associated factor 1,EDAF-1,Enterocyte differentiation-promoting factor 1,EDPF-1,MMS2 homolog,Vitamin D3-inducible protein

Bendless-like ubiquitin-conjugating enzyme,E2 ubiquitin-conjugating enzyme N,Ubc13,UbcH13,Ubiquitin carrier protein N,Ubiquitin-protein ligase N

0.1 M bicine Trizma base pH 8.5, 12.5% PEG 1000, 12.5% PEG 3350, 12.5% MPD, 0.2 % of each Anesthetic alkaloids (lidocaine HCl H2O, procaine HCl, proparacaine HCl, tetracaine HCl)

Medical Research Council (MRC, United Kingdom)

7BBD is another structure with the same citation

Ubiquitin-conjugating enzyme E2 variant 2, Ubiquitin-conjugating enzyme E2 N (E.C.2.3.2.23), Polyubiquitin-C

E2 conjugating enzyme, ubiquitin chain elongation, LIGASE

(chain A and (resid 4 through 9 or (resid 10 and (name N or name CA or name C or name O or name CB )) or resid 11 through 23 or (resid 24 through 25 and (name N or name CA or name C or name O or name CB )) or resid 26 through 28 or (resid 29 and (name N or name CA or name C or name O or name CB )) or resid 30 through 47 or (resid 48 and (name N or name CA or name C or name O or name CB )) or resid 49 through 81 or (resid 82 and (name N or name CA or name C or name O or name CB )) or resid 83 through 122 or resid 124 through 127 or resid 129 or resid 131 through 132 or (resid 133 through 134 and (name N or name CA or name C or name O or name CB )) or resid 135 through 137 or (resid 138 and (name N or name CA or name C or name O or name CB )) or resid 139 through 140 or (resid 141 through 142 and (name N or name CA or name C or name O or name CB )) or resid 143 through 144 or (resid 145 and (name N or name CA or name C or name O or name CB )) or resid 146 through 150 or (resid 151 through 152 and (name N or name CA or name C or name O or name CB ))))

(chain D and (resid 4 through 23 or (resid 24 through 25 and (name N or name CA or name C or name O or name CB )) or resid 26 through 28 or (resid 29 and (name N or name CA or name C or name O or name CB )) or resid 30 through 47 or (resid 48 and (name N or name CA or name C or name O or name CB )) or resid 49 through 59 or (resid 60 and (name N or name CA or name C or name O or name CB )) or resid 61 through 117 or (resid 118 and (name N or name CA or name C or name O or name CB )) or resid 119 through 122 or resid 124 through 127 or resid 129 or resid 131 through 137 or (resid 138 and (name N or name CA or name C or name O or name CB )) or resid 139 through 140 or (resid 141 through 142 and (name N or name CA or name C or name O or name CB )) or resid 143 through 144 or (resid 145 and (name N or name CA or name C or name O or name CB )) or resid 146 through 149 or (resid 150 through 152 and (name N or name CA or name C or name O or name CB ))))

(chain G and (resid 4 through 9 or (resid 10 and (name N or name CA or name C or name O or name CB )) or resid 11 through 59 or (resid 60 and (name N or name CA or name C or name O or name CB )) or resid 61 through 81 or (resid 82 and (name N or name CA or name C or name O or name CB )) or resid 83 through 117 or (resid 118 and (name N or name CA or name C or name O or name CB )) or resid 119 through 122 or resid 124 through 127 or resid 129 or resid 131 through 132 or (resid 133 through 134 and (name N or name CA or name C or name O or name CB )) or resid 135 through 149 or (resid 150 through 152 and (name N or name CA or name C or name O or name CB ))))

(chain B and ((resid 5 and (name N or name CA or name C or name O or name CB )) or resid 6 through 19 or (resid 20 and (name N or name CA or name C or name O or name CB )) or resid 21 through 54 or (resid 55 and (name N or name CA or name C or name O or name CB )) or resid 56 through 71 or (resid 72 and (name N or name CA or name C or name O or name CB )) or resid 73 through 132 or (resid 133 and (name N or name CA or name C or name O or name CB )) or resid 134 through 145))

(chain E and ((resid 5 and (name N or name CA or name C or name O or name CB )) or resid 6 through 19 or (resid 20 and (name N or name CA or name C or name O or name CB )) or resid 21 through 54 or (resid 55 and (name N or name CA or name C or name O or name CB )) or resid 56 through 132 or (resid 133 and (name N or name CA or name C or name O or name CB )) or resid 134 through 145))

(chain H and (resid 5 through 71 or (resid 72 and (name N or name CA or name C or name O or name CB )) or resid 73 through 145))

(chain C and (resid 1 through 59 or resid 61 through 72 or (resid 73 and (name N or name CA or name C or name O or name CB ))))

(chain F and (resid 1 through 5 or (resid 6 and (name N or name CA or name C or name O or name CB )) or resid 7 through 23 or (resid 24 and (name N or name CA or name C or name O or name CB )) or resid 25 through 32 or (resid 33 and (name N or name CA or name C or name O or name CB )) or resid 34 through 39 or (resid 40 and (name N or name CA or name C or name O or name CB )) or resid 41 through 59 or resid 61 through 73))

(chain I and (resid 1 through 5 or (resid 6 and (name N or name CA or name C or name O or name CB )) or resid 7 through 23 or (resid 24 and (name N or name CA or name C or name O or name CB )) or resid 25 through 32 or (resid 33 and (name N or name CA or name C or name O or name CB )) or resid 34 through 39 or (resid 40 and (name N or name CA or name C or name O or name CB )) or resid 41 through 59 or resid 61 through 71 or (resid 72 through 73 and (name N or name CA or name C or name O or name CB ))))