Solution structure of Rap1b/talin complex

Structure of Rap1b bound to talin reveals a pathway for triggering integrin activation.

National Institutes of Health/National Heart, Lung, and Blood Institute (NIH/NHLBI)

20 mM NaH2PO4/Na2HPO4 (pH 6.6), 50 mM NaCl, 5 mM MgCl2, 2 mM d-DTT, and 5% D2O

20 mM NaH2PO4/Na2HPO4 (pH 6.6), 50 mM NaCl, 5 mM MgCl2, 2 mM d-DTT, and 99.8% D2O

15N/13C-labeled Rap1b in the presence of unlabeled talin-F0

15N/13C- labeled talin-F0 in the presence of unlabeled Rap1b

15N/100% 2H-labeled talin-F0 in the presence of unlabeled Rap1b

15N/13C-labeled talin-F0 in the presence of unlabeled Rap1b prepared in 99.8% D2O

Schwieters, Kuszewski, Tjandra and Clore

Complex, Small GTPase, ubiquitin-like fold, CELL ADHESION