Structure of 30S (S1 depleted) ribosomal subunit and RNA polymerase complex

Structure of RNA polymerase bound to ribosomal 30S subunit.

20 mM Tris-HCl, pH 7.0, 100 mM NH4Cl, 10 mM MgCl2, 0.5 mM EDTA, 6 mM BME

30S (S1 depleted) ribosomal subunit and RNA polymerase complex

The average electron dose 40.0 (e-/A2) represents the total dose for one collected movie.

2.5 uL of 50 nM 30S and 150 nM RNAP was applied to the grid. After a 30 second incubation, the grid was blotted for 5 seconds at blotting power 8.

DNA-directed RNA polymerase subunit alpha

DNA-directed RNA polymerase subunit beta

DNA-directed RNA polymerase subunit beta'

DNA-directed RNA polymerase subunit omega

RNAP subunit alpha, RNA polymerase subunit alpha, Transcriptase subunit alpha

RNAP subunit beta, RNA polymerase subunit beta, Transcriptase subunit beta

RNAP subunit beta', RNA polymerase subunit beta', Transcriptase subunit beta'

RNAP omega subunit, RNA polymerase omega subunit, Transcriptase subunit omega

National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
Structure of 30S ribosomal subunit and RNA polymerase complex in non-rotated state
Structure of 30S ribosomal subunit and RNA polymerase complex in rotated state
DNA-directed RNA polymerase subunit alpha, DNA-directed RNA polymerase subunit beta, DNA-directed RNA polymerase subunit beta', DNA-directed RNA polymerase subunit omega, 30S ribosomal protein S2, 30S ribosomal protein S3, 30S ribosomal protein S4, 30S ribosomal protein S5, 30S ribosomal protein S6, 30S ribosomal protein S7, 30S ribosomal protein S8, 30S ribosomal protein S9, 30S ribosomal protein S10, 30S ribosomal protein S11, 30S ribosomal protein S12, 30S ribosomal protein S13, 30S ribosomal protein S14, 30S ribosomal protein S15, 30S ribosomal protein S16, 30S ribosomal protein S17, 30S ribosomal protein S18, 30S ribosomal protein S19, 30S ribosomal protein S20, 30S ribosomal protein S21/DNA Complex
30S subunit, RNA polymerase, complex, RIBOSOME