Arthrobacter globiformis T6 isomalto-dextranse

Crystal Structure and Mutational Analysis of Isomalto-dextranase, a Member of Glycoside Hydrolase Family 27

The sequence for the mature enzyme "ATAV ... ELDM" (606 amino acid residues) was fused with N-terminal His-tag, and the expressed protein was digested with thrombin. Therefore, the first four N-terminal residues GSHM are derived from the expression plasmid pET28a.

PEG 8000, sodium acetate buffer, potassium dihydrogenphosphate

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS

TIM barrel, glycoside hydrolase, GH27, carbohydrate binding module, CBM35, HYDROLASE