Structure of the mixed-function P450 MycG F286V mutant in complex with mycinamicin V in P1 space group

Substrate Recognition by the Multifunctional Cytochrome P450 Mycg in Mycinamicin Hydroxylation and Epoxidation Reactions.

1.6 M AMMONIUM SULFATE, 0.1 M MES PH6.5, 10% V/V 1,4-DIOXANE

STRUCTURE OF THE MIXED-FUNCTION P450 MYCG IN COMPLEX WITH MYCINAMICIN IV

STRUCTURE OF THE MIXED-FUNCTION P450 MYCG IN COMPLEX WITH MYCINAMICIN V IN P21 SPACE GROUP

MIXED-FUNCTION P450 MYCG IN COMPLEX WITH MYCINAMICIN III IN C2221 SPACE GROUP

STRUCTURE OF THE MIXED-FUNCTION P450 MYCG IN COMPLEX WITH MYCINAMICIN IV IN P21212 SPACE GROUP

MIXED-FUNCTION P450 MYCG IN COMPLEX WITH MYCINAMICIN III IN P21212 SPACE GROUP

STRUCTURE OF THE MIXED-FUNCTION P450 MYCG IN P21 SPACE GROUP

STRUCTURE OF THE MIXED-FUNCTION P450 MYCG F286A MUTANT IN COMPLEX WITH MYCINAMICIN IV

ENGINEERED RESIDUE IN CHAIN A, PHE 286 TO VAL ENGINEERED RESIDUE IN CHAIN B, PHE 286 TO VAL

MYCINAMICIN V (MYV): NATIVE MYCG SUBSTRATE
PROTOPORPHYRIN IX CONTAINING FE (HEM): HEME THIOLATE BOND
TO CYS 346
GLYCEROL (GOL): CRYO-PROTECTANT

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS.

OXIDOREDUCTASE, MYCINAMICIN BIOSYNTHESIS