Crystal structure of C. crescentus PNPase bound to RNA

Crystal Structure of Caulobacter Crescentus Polynucleotide Phosphorylase Reveals a Mechanism of RNA Substrate Channelling and RNA Degradosome Assembly.

POLYRIBONUCLEOTIDE NUCLEOTIDYLTRANSFERASE

RNA, 5'-R(*UP*AP*AP*CP*UP*UP*UP*GP*GP)-3'
CO-PURIFIED RNA FROM E. COLI EXPRESSION STRAIN

24% WT/V PEG 3350, 0.1 M BIS-TRIS PH 5.5, 0.1 M AMMONIUM ACETATE

CRYSTAL STRUCTURE OF C. CRESCENTUS PNPASE BOUND TO RNASE E RECOGNITION PEPTIDE

ADENOSINE-5'-MONOPHOSPHATE (A): H1 DISORDERED AND BASE ONLY
IS MODELLED
PHOSPHATE ION (PO4): TWO PHOSPHATES MOLECULES MODELLED AT
EACH ACTIVE SITE
URIDINE-5'-MONOPHOSPHATE (U): E7, D1, I1 DISORDERED AND
BASE ONLY IS MODELLED

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS. RNA CHAIN E RESIDUE 7 IS DISORDERED AND BASE IS NOT MODELLED RNA CHAINS D I AND H ARE DISORDERED AND BASE ONLY IS MODELLED. S1 DOMAINS RESIDUES 623-712 WERE NOT MODELLED

POLYRIBONUCLEOTIDE NUCLEOTIDYLTRANSFERASE (E.C.2.7.7.8)

TRANSFERASE-RNA COMPLEX, KH DOMAIN, RNASE E