CO-bound [FeFe]-hydrogenase I from Clostridium pasteurianum (CpI) at 1.34 Angstrom

Binuclear [FeFe], di(thiomethyl)amine, carbon monoxide, cyanide cluster (-CO form)

Cyanide Binding to [FeFe]-Hydrogenase Stabilizes the Alternative Configuration of the Proton Transfer Pathway.

PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)

CpI,Fe-only hydrogenase,[Fe] hydrogenase

0.1M Mes-NaOH (pH6.0), 0.4M MgCl2, 19% PEG4000, 21% glycerol

The CO is bound to the active center in the new structure

[FeFe]-hydrogenase, Clostridium pasteurianum, CO-bound, OXIDOREDUCTASE