Crystal Structure of N-acetylneuraminic acid lyase mutant K165C from Staphylococcus aureus

Structural Insights Into the Recovery of Aldolase Activity in N-Acetylneuraminic Acid Lyase by Replacement of the Catalytically Active Lysine with Gamma-Thialysine by Using a Chemical Mutagenesis Strategy.

N-ACETYLNEURAMINIC ACID LYASE, N-ACETYLNEURAMINATE PYRUVATE-LYASE, N-ACETYLNEURAMINIC ACID ALDOLASE, SIALATE LYASE, SIALIC ACID ALDOLASE, SIALIC ACID LYASE

STAPHYLOCOCCUS AUREUS SUBSP. AUREUS NCTC 8325

200MM NACL, 100MM TRIS-HCL PH7.4, 28% PEG 3350

CRYSTAL STRUCTURE OF N-ACETYLNEURAMINIC ACID LYASE FROM STAPHYLOCOCCUS AUREUS WITH THE CHEMICAL MODIFICATION THIA-LYSINE AT POSITION 165

STRUCTURE OF WILD TYPE STAPYLOCOCCUS AUREUS N- ACETYLNEURAMINIC ACID LYASE IN COMPLEX WITH PYRUVATE

CRYSTAL STRUCTURE OF WILD TYPE N-ACETYLNEURAMINIC ACID LYASE FROM STAPHYLOCOCCUS AUREUS

CRYSTAL STRUCTURE OF N-ACETYLNEURAMINIC ACID LYASE WITH THE UNNATURAL AMINO ACID THIALYSINE AT POSITION 165 IN COMPLEX WITH PYRUVATE

SHEET
DETERMINATION METHOD: DSSP
THE SHEETS PRESENTED AS "AA" IN EACH CHAIN ON SHEET RECORDS
BELOW IS ACTUALLY AN 8-STRANDED BARREL THIS IS REPRESENTED BY
A 9-STRANDED SHEET IN WHICH THE FIRST AND LAST STRANDS
ARE IDENTICAL.

THE SHEETS PRESENTED AS "BA" IN EACH CHAIN ON SHEET RECORDS
BELOW IS ACTUALLY AN 8-STRANDED BARREL THIS IS REPRESENTED BY
A 9-STRANDED SHEET IN WHICH THE FIRST AND LAST STRANDS
ARE IDENTICAL.

THE SHEETS PRESENTED AS "CA" IN EACH CHAIN ON SHEET RECORDS
BELOW IS ACTUALLY AN 8-STRANDED BARREL THIS IS REPRESENTED BY
A 9-STRANDED SHEET IN WHICH THE FIRST AND LAST STRANDS
ARE IDENTICAL.

THE SHEETS PRESENTED AS "DA" IN EACH CHAIN ON SHEET RECORDS
BELOW IS ACTUALLY AN 8-STRANDED BARREL THIS IS REPRESENTED BY
A 9-STRANDED SHEET IN WHICH THE FIRST AND LAST STRANDS
ARE IDENTICAL.

ENGINEERED RESIDUE IN CHAIN A, LYS 165 TO CYS ENGINEERED RESIDUE IN CHAIN B, LYS 165 TO CYS ENGINEERED RESIDUE IN CHAIN C, LYS 165 TO CYS ENGINEERED RESIDUE IN CHAIN D, LYS 165 TO CYS

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS.