RdeltaD2 H/L (LMW SLP/HMW SLP) complex from C. difficile SlpA (R20291 strain)

Structure and assembly of the S-layer in C. difficile.

SLPL deltaD2 (LMW SLP D2 truncation),SLPL deltaD2 (LMW SLP D2 truncation)
SLPL deltaD2 (LMW SLP D2 truncation) from C. difficile (R20291),SLPL deltaD2 (LMW SLP D2 truncation)

SLPH (HMW SLP) from C. difficile (R20291)

Cell surface protein (S-layer protein),Cell surface protein (S-layer protein)

Clostridioides difficile (strain R20291)

0.1 M MES pH 6.0
0.875 M Lithium chloride
11.5% PEG 6,000
10% Glycerol

ELISA assay probed SLPL and SLPH interaction and confirmed H/L complex formation (Fagan et al, Mol. Cell Biol., 2019)

The Rmerge is very high for this crystal due to merging of data from 2 highly anisotropic crystals, that are also not completely isomorphous. However, this was necessary to generate interpretable electron density maps, as data from either crystal separately was enough.

Bacterial surface, S-layer, STRUCTURAL PROTEIN

(chain "A" and (resid 5 or (resid 6 and (name N or name CA or name C or name O or name CB )) or resid 7 through 15 or (resid 16 and (name N or name CA or name C or name O or name CB )) or resid 17 through 22 or (resid 23 through 24 and (name N or name CA or name C or name O or name CB )) or resid 25 through 29 or (resid 30 and (name N or name CA or name C or name O or name CB )) or resid 31 through 33 or (resid 34 and (name N or name CA or name C or name O or name CB )) or resid 35 through 44 or (resid 45 and (name N or name CA or name C or name O or name CB )) or resid 46 through 54 or resid 59 through 64 or (resid 65 and (name N or name CA or name C or name O or name CB )) or resid 66 through 71 or (resid 72 and (name N or name CA or name C or name O or name CB )) or resid 73 through 81 or (resid 82 and (name N or name CA or name C or name O or name CB )) or resid 83 through 90 or resid 98 through 106 or (resid 107 through 109 and (name N or name CA or name C or name O or name CB )) or resid 110 through 111 or (resid 112 and (name N or name CA or name C or name O or name CB )) or resid 113 or (resid 114 through 115 and (name N or name CA or name C or name O or name CB )) or resid 119 through 131 or (resid 132 and (name N or name CA or name C or name O or name CB )) or resid 133 through 144 or (resid 153 through 159 and (name N or name CA or name C or name O or name CB )) or resid 160 or (resid 161 and (name N or name CA or name C or name O or name CB )) or resid 162 through 172))

(chain "C" and (resid 5 through 60 or (resid 61 through 63 and (name N or name CA or name C or name O or name CB )) or resid 64 through 136 or (resid 137 and (name N or name CA or name C or name O or name CB )) or resid 138 through 172))

(chain "B" and ((resid 3 through 4 and (name N or name CA or name C or name O or name CB )) or resid 5 or (resid 6 through 7 and (name N or name CA or name C or name O or name CB )) or resid 8 through 17 or (resid 18 and (name N or name CA or name C or name O or name CB )) or resid 19 through 22 or (resid 23 through 24 and (name N or name CA or name C or name O or name CB )) or resid 25 or (resid 26 through 27 and (name N or name CA or name C or name O or name CB )) or resid 28 through 30 or (resid 31 and (name N or name CA or name C or name O or name CB )) or resid 32 through 69 or (resid 70 and (name N or name CA or name C or name O or name CB )) or resid 71 or (resid 72 through 73 and (name N or name CA or name C or name O or name CB )) or resid 74 through 87 or (resid 88 and (name N or name CA or name C or name O or name CB )) or resid 89 or (resid 90 through 92 and (name N or name CA or name C or name O or name CB )) or resid 93 through 98 or (resid 99 through 101 and (name N or name CA or name C or name O or name CB )) or resid 102 through 126 or (resid 127 and (name N or name CA or name C or name O or name CB )) or resid 128 through 183 or resid 186 through 189 or (resid 190 and (name N or name CA or name C or name O or name CB )) or resid 191 through 192 or (resid 193 and (name N or name CA or name C or name O or name CB )) or resid 194 through 213 or (resid 214 and (name N or name CA or name C or name O or name CB )) or resid 215 through 233 or (resid 234 through 236 and (name N or name CA or name C or name O or name CB )) or resid 237 through 262 or (resid 263 through 265 and (name N or name CA or name C or name O or name CB )) or resid 266 or (resid 267 through 268 and (name N or name CA or name C or name O or name CB )) or resid 269 through 281 or (resid 282 through 283 and (name N or name CA or name C or name O or name CB )) or resid 284 through 296 or (resid 297 through 298 and (name N or name CA or name C or name O or name CB )) or resid 299 through 305 or (resid 306 and (name N or name CA or name C or name O or name CB )) or resid 307 through 317 or (resid 318 through 321 and (name N or name CA or name C or name O or name CB )) or resid 322 through 340 or (resid 341 and (name N or name CA or name C or name O or name CB )) or resid 342 through 345 or (resid 346 through 348 and (name N or name CA or name C or name O or name CB )) or resid 349 or (resid 357 through 359 and (name N or name CA or name C or name O or name CB )) or resid 360 through 411 or (resid 412 and (name N or name CA or name C or name O or name CB )) or resid 413 through 416))

(chain "D" and (resid 3 through 19 or (resid 20 and (name N or name CA or name C or name O or name CB )) or resid 21 through 47 or (resid 48 through 49 and (name N or name CA or name C or name O or name CB )) or resid 50 through 99 or (resid 100 through 101 and (name N or name CA or name C or name O or name CB )) or resid 102 through 107 or (resid 108 and (name N or name CA or name C or name O or name CB )) or resid 109 or (resid 110 and (name N or name CA or name C or name O or name CB )) or resid 111 through 147 or (resid 148 and (name N or name CA or name C or name O or name CB )) or resid 149 through 154 or (resid 155 and (name N or name CA or name C or name O or name CB )) or resid 156 through 182 or (resid 183 through 187 and (name N or name CA or name C or name O or name CB )) or resid 188 through 196 or (resid 197 through 198 and (name N or name CA or name C or name O or name CB )) or resid 199 through 200 or (resid 201 through 202 and (name N or name CA or name C or name O or name CB )) or resid 203 through 215 or (resid 216 and (name N or name CA or name C or name O or name CB )) or resid 217 through 218 or (resid 219 and (name N or name CA or name C or name O or name CB )) or resid 220 through 239 or (resid 240 and (name N or name CA or name C or name O or name CB )) or resid 241 through 259 or (resid 260 and (name N or name CA or name C or name O or name CB )) or resid 261 through 316 or (resid 317 through 321 and (name N or name CA or name C or name O or name CB )) or resid 322 through 346 or (resid 347 through 348 and (name N or name CA or name C or name O or name CB )) or resid 349 or (resid 357 through 359 and (name N or name CA or name C or name O or name CB )) or resid 360 through 386 or (resid 387 and (name N or name CA or name C or name O or name CB )) or resid 388 through 408 or (resid 409 and (name N or name CA or name C or name O or name CB )) or resid 410 through 416))