Partial dimer complex of PAPP-A and its inhibitor STC2

N-acetyl-beta-D-glucosamine; 2-acetamido-2-deoxy-beta-D-glucose; 2-acetamido-2-deoxy-D-glucose; 2-acetamido-2-deoxy-glucose; N-ACETYL-D-GLUCOSAMINE

Structure of the proteolytic enzyme PAPP-A with the endogenous inhibitor stanniocalcin-2 reveals its inhibitory mechanism.

alphafold2 model
PAPP-A AF-Q13219-F1
STC2 AF-O76061-F1

Hepes buffer,
20 mM Hepes pH 7.4 100 mM NaCl, 1 mM CaCl

Inhibited proteolytic complex generated by harvest of serum media, purifying on a nickel column followed by negative affinity purification and size-exclusion chromatography.
Partial PAPP-A dimer in complex with subunit of its endogenous inhibitor STC2 dimer

The first approximately 1000 residues of a PAPP-A subunit

The C-terminal 240 residues of one PAPP-A subunit

Insulin-like growth factor-dependent IGF-binding protein 4 protease,IGF-dependent IGFBP-4 protease,IGFBP-4ase,Pregnancy-associated plasma protein A,PAPP-A

STC-2,Stanniocalcin-related protein,STC-related protein,STCRP

Metzincin metalloprotease Inhibitor complex, HYDROLASE