Crystal structure of the C258S/C268S variant of Toxoplasma gondii nucleoside triphosphate diphosphohydrolase 3 (NTPDase3) in complex with magnesium and AMPPNP

PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER

Structural Insight Into the Activation Mechanism of Toxoplasma Gondii Nucleoside Triphosphate Diphosphohydrolases by Disulfide Reduction.

NTPASE-I, NUCLEOSIDE TRIPHOSPHATE HYDROLASE 1, NUCLEOSIDE-TRIPHOSPHATASE I, NTPDASE3

100 MM KCL, 10 MM MGCL2, 50 MM TRIS PH 8.5, 30% PEG 400, 2 MM AMPPNP AND 10 MM MAGNESIUM ACETATE.

CRYSTAL STRUCTURE OF TOXOPLASMA GONDII NUCLEOSIDE TRIPHOSPHATE DIPHOSPHOHYDROLASE 3 (NTPDASE3)

CRYSTAL STRUCTURE OF TOXOPLASMA GONDII NUCLEOSIDE TRIPHOSPHATE DIPHOSPHOHYDROLASE 3 (NTPDASE3) IN COMPLEX WITH AMP

CRYSTAL STRUCTURE OF THE C258S/C268S VARIANT OF TOXOPLASMA GONDII NUCLEOSIDE TRIPHOSPHATE DIPHOSPHOHYDROLASE 1 (NTPDASE1)

ENGINEERED RESIDUE IN CHAIN A, CYS 258 TO SER ENGINEERED RESIDUE IN CHAIN A, CYS 268 TO SER ENGINEERED RESIDUE IN CHAIN B, CYS 258 TO SER ENGINEERED RESIDUE IN CHAIN B, CYS 268 TO SER ENGINEERED RESIDUE IN CHAIN C, CYS 258 TO SER ENGINEERED RESIDUE IN CHAIN C, CYS 268 TO SER ENGINEERED RESIDUE IN CHAIN D, CYS 258 TO SER ENGINEERED RESIDUE IN CHAIN D, CYS 268 TO SER

HYDROGENS HAVE BEEN USED IF PRESENT IN THE INPUT.

NUCLEOSIDE-TRIPHOSPHATASE 1 (E.C.3.6.1.5)