Assignment of 1H,13C and 15N resonances for the REF2-I mRNA export factor

REF2-I double domain, including N-terminal and RRM domains (residues 1-155), with additional N-terminal T7 tag and C-terminal His6-tag.

The first and second authors contributed equally to this publication.
Assignment of 1H, 13C, and 15N resonances for the REF2-I mRNA export factor.

Journal of the American Chemical Society
A simple method for improving protein solubility and long-term stability.

-13 MET
-12 ALA
-11 SER
-10 MET
-9 THR
-8 GLY
-7 GLY
-6 GLN
-5 GLN
-4 MET
-3 GLY
-2 ARG
-1 ASP
0 PRO
1 MET
2 ALA
3 ASP
4 LYS
5 MET
6 ASP
7 MET
8 SER
9 LEU
10 ASP
11 ASP
12 ILE
13 ILE
14 LYS
15 LEU
16 ASN
17 ARG
18 ASN
19 GLN
20 ARG
21 ARG
22 VAL
23 ASN
24 ARG
25 GLY
26 GLY
27 GLY
28 PRO
29 ARG
30 ARG
31 ASN
32 ARG
33 PRO
34 ALA
35 ILE
36 ALA
37 ARG
38 GLY
39 GLY
40 ARG
41 ASN
42 ARG
43 PRO
44 ALA
45 PRO
46 TYR
47 SER
48 ARG
49 PRO
50 LYS
51 PRO
52 LEU
53 PRO
54 ASP
55 LYS
56 TRP
57 GLN
58 HIS
59 ASP
60 LEU
61 PHE
62 ASP
63 SER
64 GLY
65 CYS
66 GLY
67 GLY
68 GLY
69 GLU
70 GLY
71 VAL
72 GLU
73 THR
74 GLY
75 ALA
76 LYS
77 LEU
78 LEU
79 VAL
80 SER
81 ASN
82 LEU
83 ASP
84 PHE
85 GLY
86 VAL
87 SER
88 ASP
89 ALA
90 ASP
91 ILE
92 GLN
93 GLU
94 LEU
95 PHE
96 ALA
97 GLU
98 PHE
99 GLY
100 THR
101 LEU
102 LYS
103 LYS
104 ALA
105 ALA
106 VAL
107 ASP
108 TYR
109 ASP
110 ARG
111 SER
112 GLY
113 ARG
114 SER
115 LEU
116 GLY
117 THR
118 ALA
119 ASP
120 VAL
121 HIS
122 PHE
123 GLU
124 ARG
125 ARG
126 ALA
127 ASP
128 ALA
129 LEU
130 LYS
131 ALA
132 MET
133 LYS
134 GLN
135 TYR
136 LYS
137 GLY
138 VAL
139 PRO
140 LEU
141 ASP
142 GLY
143 ARG
144 PRO
145 MET
146 ASP
147 ILE
148 GLN
149 LEU
150 VAL
151 ALA
152 SER
153 GLN
154 ILE
155 ASP
156 LEU
157 GLU
158 HIS

14-residue N-terminal T7 tag (MASMTGGQQMGRDP) was added to the sequence of
protein. C-terminal His6-tag with linker region is also added (LEHHHHHH);
signals from five out of six histidins are invisible in the spectra and hence
are not assigned.

The Solution Structure Of The Ref2-I Mrna Export Factor (Residues 1-155)

Rrm Domain Of Mrna Export Adaptor Ref2-i Bound To Hvs Orf57 Peptide

RNA and export factor binding protein 2-I [Mus musculus]

RNA and export factor binding protein 2 [Mus musculus]

RecName: Full=Aly/REF export factor 2; AltName: Full=Alyref; AltName: Full=RNA and export factor-binding protein 2 [Mus musculu

The 50 mM L-Arg and 50 mM L-Glu were added to improve protein solubility and sample stability, see Reference 1.