Assignment of 1H, 13C and 15N resonances of barnacle cement protein Mrcp-20k

NMR assignment of the barnacle cement protein mrcp-20k.

Hyperfine shifted 1H signals in a range of 55-75 ppm are oberved in 1D 1H
spectra of Mrcp20k suggesting the existence of paramagnetic metal atom(s) that
is not specified.

The MS measurements together with the chemical modification experiments
indicate that all the Cys residues participate in intramolecular S-S
bond formation. But the disulfide bond linkages have not been
experimentally determined because of the abundance of the cystein
residues in the protein.