Three-disulfide variant of hen lysozyme: C64A/C80A

NMR characterization of three-disulfide variants of lysozyme, C64A/C80A, C76A/C94A, and C30A/C115A--a marginally stable state in folded proteins.

Reduced denatured lysozyme has been oxidised and refolded at pH values close to
neutral in an efficient way by dilution from buffers containing 8.0 M urea, and
refolding intermediates were separated by reverse-phase HPLC at pH 2. By using
peptic digestion in combination with high-resolution Fourier transform ion
cyclotron resonance (FT-ICR) mass spectrometry (MS) and tandem MS/MS the
dominant intermediate was identified to be des-[76-94]. This species has three
of the four native disulphide bonds, but lacks the Cys76-Cys94 disulphide bond
which connects the two folding domains in the native protein. Characterisation
of des-[76-94] by 2D1H NMR shows that it has a highly native-like structure.
This provides an explanation for the accumulation of this species during
refolding as direct oxidation to the fully native protein will be restricted by
the burial of Cys94 in the protein interior.

van den Berg B, Chung EW, Robinson CV, Dobson CM.
Characterisation of the dominant oxidative folding intermediate of hen
lysozyme.
J Mol Biol. 1999 Jul 16;290(3):781-96.

Characterisation of the dominant oxidative folding intermediate of hen lysozyme.

The 15N-labeled recombinant hen lysozyme and two species of two-disulfide
variants, denoted as 2SS[6-127, 30-115] and 2SS[64-80, 76-94], were studied by
means of NMR spectroscopy. The former variant contains two disulfide bridges in
the alpha-domain, while the latter has one disulfide bridge in the beta-domain
and the other one at the interface between two domains. Resonance assignments
were performed using 3D TOCSY-HSQC and NOESY-HSQC spectra. The 15N-1H-HSQC
spectrum of 2SS[6-127, 30-115] was similar to that of recombinant lysozyme as a
whole, although a number of cross-peaks disappeared. On the other hand, the
HSQC spectrum of 2SS[64-80, 76-94] was characteristic of unfolded proteins. The
structure of 2SS[6-127, 30-115] was thoroughly examined on the basis of NOE
contacts determined by NMR spectroscopy. The structure of the alpha-domain was
quite similar to that of authentic lysozyme, while the beta-domain was largely
unstructured. However, NMR data clearly demonstrated that some residual
structures exist in the beta-domain. The beta1 and beta2 strands were
maintained stably as an antiparallel beta-sheet. In addition, the residues 55
and 56 were located in the vicinity of the end of the B-helix. Further, the
C-helix was properly set with side-chains of I88, V92, K96, and V99 facing
toward the hydrophobic core in the alpha-domain. These residual structures
inherent in the amino acid sequence were evaluated concerning the folding
process of lysozyme. Our experiments imply that the establishment of the
backbone conformation ranging from residues 76-99 plays a key role in attaining
the cooperativity between two domains required for the folding transition.

Noda Y, Yokota A, Horii D, Tominaga T, Tanisaka Y, Tachibana H, Segawa S.
NMR structural study of two-disulfide variant of hen lysozyme: 2SS[6-127,
30-115]--a disulfide intermediate with a partly unfolded structure.
Biochemistry. 2002 Feb 19;41(7):2130-9.

NMR structural study of two-disulfide variant of hen lysozyme: 2SS[6-127, 30-115]--a disulfide intermediate with a partly unfolded structure.

Hen lysozyme with Cys64 and Cys80 replaced by Ala and three disulfide bridges
between Cys6 and Cys127, between Cys30 and Cys115, and between Cys76 and Cys94.

Lysozyme C precursor (1,4-beta-N-acetylmuramidase C) (Allergen Gal d 4) (Allergen Gal d IV)

An X-Ray Study Of The Structure And Binding Properties Of Iodine-Inactivated Lysozyme

Protein Model Building By The Use Of A Constrained- Restrained Least-Squares Procedure

Real-Space Refinement Of The Structure Of Hen Egg-White Lysozyme

Comparison Of Radiation-Induced Decay And Structure Refinement From X-Ray Data Collected From Lysozyme Crystals At Low And Ambient Temperatures

Studies Of Monoclinic Hen Egg White Lysozyme. Iv. X-Ray Refinement At 1.8 Angstrom Resolution And A Comparison Of The Variable Regions In The Polymorphic Forms

Atomic Resolution Refinement Of Triclinic Hew Lysozyme At 295k

Cross-Linked Chicken Lysozyme Crystal In Neat Acetonitrile, Then Back-Soaked In Water

Crystal Structure Of Low Humidity Tetragonal Lysozyme At 2.1-Angstroms Resolution. Variability In Hydration Shell And Its Structural Consequences

Refinement Of Triclinic Lysozyme At Atomic Resolution

Cross-Linked Chicken Lysozyme Crystal In 95% Acetonitrile- Water

Crystal Structure Of Hen Egg-White Lysozyme At A Hydrostatic Pressure Of 1000 Atmospheres

Structure Of An Antibody-Antigen Complex. Crystal Structure Of The HyHEL-10 Fab-Lysozyme Complex

High Resolution Crystal Structure Structure Of Hyhel10 Fab Complexed To Hen Egg Lysozyme

Crystal Structure Of Lysozyme Folded In Sds And 2-Methyl-2, 4-Pentanediol

Phase Transition Of Monoclinic Lysozyme Crystal Soaked In A Saturated Nacl Solution

Phase Transition Of Monoclinic Lysozyme Crystal Soaked In A 10% Nacl Solution

Structure Of The Transformed Monoclinic Lysozyme By Controlled Dehydration

High Resolution X-Ray Crystal Structure Of Tetragonal Hen Egg White Lysozyme

Crystal Structure Of Humanized Hyhel-10 Fv Mutant(Hq39kw47y)-Hen Lysozyme Complex

Tricarbonylmanganese(I)-Lysozyme Complex : A Structurally Characterized Organometallic Protein

Lysozyme Cocrystallized With Tris-Dipicolinate Eu Complex

Refinement Of Triclinic Lysozyme. Ii. The Method Of Stereochemically Restrained Least-Squares

The Structures Of The Monoclinic And Orthorhombic Forms Of Hen Egg-White Lysozyme At 6 Angstroms Resolution.

Cross-Linked Chicken Lysozyme Crystal In 90% Acetonitrile- Water

X-Ray Diffraction Studies Of Adducts Between Anticancer Platinum Drugs And Hen Egg White Lysozyme

Crystal Structure Analysis Of The Nurse Shark New Antigen Receptor Ancestral Variable Domain In Complex With Lysozyme

Crystal Structure Analysis Of The Nurse Shark New Antigen Receptor Pbla8 Variable Domain In Complex With Lysozyme

Structure Of Hen Egg-White Lysozyme Determined From Crystals Grown In Ph 7.5

Parent Structure Of Hen Egg White Lysozyme Grown In Acidic Ph 4.8. Refinement For Comparison With Crosslinked Molecules Of Lysozyme

Crystal Structure Analysis Of Hen Egg White Lyszoyme

Crystal Structure Analysis Of Hen Egg White Lysozyme Crosslinked By Polymerized Glutaraldehyde In Acidic Environment

Multipattern Rietveld Refinement With Protein Powder Data: An Approach To Higher Resolution

Structure Of Hen Egg White Lysozyme Soaked With Ni-Cyclam

Structure Of Hen Egg White Lysozyme Soaked With Ni2- Xylylbicyclam

Anomalous Substructure Of Lysozyme At Ph 8.0

Anomalous Substructure Of Lysozyme At Ph 4.5

Crystal Structure Analysis Of Hexagonal Lysozyme

Triclinic Cross-Linked Lysozyme Soaked In Bromoethanol 1m

Triclinic Cross-Linked Lysozyme Soaked With Thiourea 1.5m

Triclinic Cross-Linked Lysozyme Soaked With 4.5m Urea

Triclinic Hen Egg Lysozyme Cross-Linked By Glutaraldehyde

Crystal Structure Analysis Of Hen Egg White Lysozyme Grown By Capillary Method

Crystal Structure Of Humanized Hyhel-10 Fv-Hen Lysozyme Complex

Crystal Structure Of Humanized Hyhel-10 Fv Mutant(Hw47y)- Hen Lysozyme Complex

Crystal Structure Of Hyhel-10 Fv (Wild-Type) Complexed With Hen Egg Lysozyme At 1.8a Resolution

Crystal Structure Of Hyhel-10 Fv Mutant (Ly50a) Complexed With Hen Egg Lysozyme

Crystal Structure Of Hyhel-10 Fv Mutant (Hy58a) Complexed With Hen Egg Lysozyme

Crystal Structure Of Hyhel-10 Fv Mutant (Hy53f) Complexed With Hen Egg Lysozyme

Crystal Structure Of Hyhel-10 Fv Mutant (Y33ay53a) Complexed With Hen Egg Lysozyme

Crystal Structure Of Hyhel-10 Fv Mutant (Hy53a) Complexed With Hen Egg Lysozyme

Crystal Structure Of Hyhel-10 Fv Mutant (Hy50f) Complexed With Hen Egg Lysozyme

Crystal Structure Of Hyhel-10 Fv Mutant(Hy33f) Complexed With Hen Egg Lysozyme

Novel Bromate Species Trapped Within A Protein Crystal

Monoclinic Hen Egg-White Lysozyme Crystallized At 313k

Transformed Monoclinic Crystal Of Hen Egg-White Lysozyme From A Heavy Water Solution

Monoclinic Hen Egg-White Lysozyme Crystallized At Ph4.5 Form Heavy Water Solution

Siras Structure Of Tetragonal Lysosyme Using Derivative Data Collected At The High Energy Remote Holmium Kedge

Lysozyme (60sec) And Uv Laser Excited Fluorescence

Lysozyme (1sec) And Uv Lasr Excited Fluorescence

The Kedge Holmium Derivative Of Hen Egg-White Lysozyme At High Resolution From Single Wavelength Anomalous Diffraction

Lysozyme Structure Derived From Thin-Film-Based Crystals

On The Routine Use Of Soft X-Rays In Macromolecular Crystallography, Part Iii- The Optimal Data Collection Wavelength

Ph Evolution Of Tetragonal Hewl At 4 Degrees Celcius.

Crystal Structure Of The Vhh D3-L11 In Complex With Hen Egg White Lysozyme

Crystal Stucture Of The Vhh Domain D2-L24 In Complex With Hen Egg White Lysozyme

Crystal Structure Of The Variable Domain Of The Camelid Heavy-Chain Antibody D2-L29 In Complex With Hen Egg White Lysozyme

Cabbcii-10 Vhh Framework With Cdr Loops Of Cablys3 Grafted On It And In Complex With Hen Egg White Lysozyme

The Crystal Structure Of The Antibody Fab Hyhel5 Complex With Lysozyme At 1.7a Resolution

Structure Of Hen Egg White Lysozyme Soaked With Cu2- Xylylbicyclam

Structure Of Hen Egg White Lysozyme Soaked With Cu-Cyclam

Structure For Antibody Hyhel-63 Y33f Mutant Complexed With Hen Egg Lysozyme

Structure For Antibody Hyhel-63 Y33l Mutant Complexed With Hen Egg Lysozyme

Structure For Antibody Hyhel-63 Y33i Mutant Complexed With Hen Egg Lysozyme

Structure For Antibody Hyhel-63 Y33v Mutant Complexed With Hen Egg Lysozyme

Structure For Antibody Hyhel-63 Y33a Mutant Complexed With Hen Egg Lysozyme

Crystal Structure Of The Cdr2 Germline Reversion Mutant Of Cab-Lys3 In Complex With Hen Egg White Lysozyme

The Crystal Structures Of Lysozyme At Very Low Levels Of Hydration

The Structure Of Hew Lysozyme Orthorhombic Crystal Growth Under A High Magnetic Field

X-Ray Structure Of Hew Lysozyme Orthorhombic Crystal Formed In The Earth's Magnetic Field

High Energy Tetragonal Lysozyme X-Ray Structure

Bound Water Molecules And Conformational Stabilization Help Mediate An Antigen-Antibody Association

The Crystal Structure Of The Orthorhombic Form Of Hen Egg White Lysozyme At 1.9 Angstroms Resolution In Space

The Crystal Structure Of The Tetragonal Form Of Hen Egg White Lysozyme At 1.7 Angstroms Resolution Under Basic Conditions In Space

The Crystal Structure Of The Tetragonal Form Of Hen Egg White Lysozyme At 1.6 Angstroms Resolution In Space

The Crystal Structure Of The Orthorhombic Form Of Hen Egg White Lysozyme At 1.5 Angstroms Resolution

The Crystal Structure Of The Monoclinic Form Of Hen Egg White Lysozyme At 1.7 Angstroms Resolution In Space

Xenon Derivative Of Hen Egg-White Lysozyme

Iodine Derivative Of Hen Egg-White Lysozyme

Triclinic Lysozyme With Low Solvent Content Obtained By Phase Transition

Triclinic Hen Lysozyme Crystallized At 313k From A D2o Solution

Analysis Of The Stabilization Of Hen Lysozyme With The Helix Dipole And Charged Side Chains

Hen Egg-White Lysozyme, Low Humidity Form

Crystal Structure Of Wild-Type Hen-Egg White Lysozyme Singly Labeled With 2',3'-Epoxypropyl Beta-Glycoside Of N- Acetyllactosamine

Crystal Structure Of Hyhel-10 Fv Mutant Sfsf Complexed With Hen Egg White Lysozyme Complex

Crystal Structure Analysis Of The Nurse Shark New Antigen Receptor (Nar) Variable Domain In Complex With Lysozyme

Half-Sandwich Arene Ruthenium(Ii)-Enzyme Complex

Crystal Structure Analysis Of The Nurse Shark New Antigen Receptor (Nar) Variable Domain In Complex With Lyxozyme

Binding Of Hexa-N-Acetylchitohexaose: A Powder Diffraction Study

Binding Of Penta-N-Acetylchitopentaose To Hew Lysozyme: A Powder Diffraction Study

Binding Of Tetra-N-Acetylchitotetraose To Hew Lysozyme: A Powder Diffraction Study

Binding Of N,N',N-Triacetylchitotriose To Hew Lysozyme: A Powder Diffraction Study

Binding Of N,N'-Diacetylchitobiose To Hew Lysozyme: A Powder Diffraction Study

Crystal Structure Of The Camelid Single Domain Antibody Cab- Lys2 In Complex With Hen Egg White Lysozyme

Crystal Structure Of The Camelid Single Domain Antibody 1d2l19 In Complex With Hen Egg White Lysozyme

Crystal Structure Of A Ubiquitin-Dependent Degradation Substrate: A Three-Disulfide Form Of Lysozyme

Crystal Structure Of Hew Lysozyme Under Pressure Of Krypton (55 Bar)

Specific Chemical And Structural Damage Caused By Intense Synchrotron Radiation To Hen Egg White Lysozyme

Structure Of The Monoclinic C2 Form Of Hen Egg-White Lysozyme At 2.0 Angstroms Resolution

Crystal Structure Analysis Of An Anti-Lysozyme Antibody

Crystal Structure Of Fab Fragment Of Antibody Hyhel-26 Complexed With Lysozyme

Para-Arsanilate Derivative Of Hen Egg-White Lysozyme

Monoclonal Antibody Fab D44.1 Raised Against Chicken Egg- White Lysozyme Complexed With Lysozyme

Crystal Structure Of A Camel Single-Domain Vh Antibody Fragment In Complex With Lysozyme

Neutron Structure Of Hen Egg-White Lysozyme

Dissection Of Protein-Carbohydrate Interactions In Mutant Hen Egg-White Lysozyme Complexes And Their Hydrolytic Activity

Anomalous Signal Of Solvent Bromines Used For Phasing Of Lysozyme

Lysozyme Phased On Anomalous Signal Of Sulfurs And Chlorines

X-Ray Structure Of A Monoclinic Form Of Hen Egg-White Lysozyme Crystallized At 313k. Comparison Of Two Independent Molecules

Cross-Linked Lysozyme Crystal In Neat Water

Three-Dimensional Structure Of The Platelet Integrin Recognition Segment Of The Fibrinogen Gamma Chain Obtained By Carrier Protein-Driven Crystallization

The Influence Of Temperature On Lysozyme Crystals. Structure And Dynamics Of Protein And Water

Hew Lysozyme: Trp...Na Cation-Pi Interaction

Protein Hydration And Water Structure: X-Ray Analysis Of A Closely Packed Protein Crystal With Very Low Solvent Content

Monoclinic Hen Egg White Lysozyme Iodide

Crystal Structure Of Monoclinic Lysozyme Grown In Presence Of 15% Trehalose

Crystal Structure Of Monoclinic Lysozyme Grown In Presence Of 10% Trehalose

Crystal Structure Of Monoclinic Lysozyme Grown In Presence 10% Sorbitol

Crystal Structure Of Monoclinic Lysozyme Grown In Presence Of 5% Glycerol

Crystal Structure Of Monoclinic Lysozyme Grown In Presence Of 10% Sucrose

Crystal Structure Of Monoclinic Lysozyme Grown At Ph 4.6

Monoclinic Hen Egg White Lysozyme, Thiocyanate Complex

Fv Mutant Y(A 50)s (Vl Domain) Of Mouse Monoclonal Antibody D1.3 Complexed With Hen Egg White Lysozyme

Fv Mutant Y(B 101)f (Vh Domain) Of Mouse Monoclonal Antibody D1.3 Complexed With Hen Egg White Lysozyme

Fv Mutant Y(B 32)a (Vh Domain) Of Mouse Monoclonal Antibody D1.3 Complexed With Hen Egg White Lysozyme

Degenerate Interfaces In Antigen-Antibody Complexes

Crystal Structure Of Orthorhombic Lysozyme Grown At Ph 4.6 In Presence Of 5% Sorbitol

Crystal Structure Of Tetragonal Lysozyme Grown In Presence Of 30% Sucrose

Crystal Structure Of Tetragonal Lysozyme Grown In Presence 20% Sorbitol

Crystal Structure Of Tetragonal Lysozyme Grown In Presence 30% Trehalose

Crystal Structure Of Tetragonal Lysozyme Grown At Ph 4.6

Binding Of N-Acetylglucosamine To Chicken Egg Lysozyme: A Powder Diffraction Study

Crystal Structure Of Hyhel-10 Fv Mutant Ls93a Complexed With Hen Egg White Lysozyme

Crystal Structure Of Hyhel-10 Fv Mutant Ls91a Complexed With Hen Egg White Lysozyme

Crystal Structure Of Hyhel-10 Fv Mutant Ly50f Complexed With Hen Egg White Lysozyme

Hydrogen And Hydration Of Hen Egg-White Lysozyme Determined By Neutron Diffraction

Structure Of Tetragonal Hen Egg White Lysozyme At 0.94 A From Crystals Grown By The Counter-Diffusion Method

Crystal Structure Of Hyhel-10 Fv Mutant(Hd32a99a)-Hen Lysozyme Complex

Crystal Structure Of Hyhel-10 Fv Mutant(Hd99a)-Hen Lysozyme Complex

Crystal Structure Of Hyhel-10 Fv Mutant(Hd32a)-Hen Lysozyme Complex

Lysozyme Grown At Basic Ph And Its Low Humidity Variant

Lysozyme (Mucopeptide N-Acetylmuramyl Hydrolase)

Structural Effects Of Monovalent Anions On Polymorphic Lysozyme Crystals

Refinement Of An Enzyme Complex With Inhibitor Bound At Partial Occupancy. Hen Egg-White Lysozyme And Tri-N- Acetylchitotriose At 1.75 Angstroms Resolution

Structural And Thermodynamic Analysis Of Compensating Mutations Within The Core Of Chicken Egg White Lysozyme

Gadolinium Derivative Of Tetragonal Hen Egg-White Lysozyme At 1.7 A Resolution

Solution Structure Of Lysozyme At Low And High Pressure

Tri-Iodide Derivative Of Hen Egg-White Lysozyme

Structure Of Ivy Complexed With Its Target, Hewl

Crystal Structure Of Hen Egg White Lysozyme (Hel) Complexed With The Mutant Anti-Hel Monoclonal Antibody D1.3 (Vlw92v)

Crystal Structure Of Hen Egg White Lysozyme (Hel) Complexed With The Mutant Anti-Hel Monoclonal Antibody D1.3 (Vlw92s)

Crystal Structure Of Hen Egg White Lysozyme (Hel) Complexed With The Mutant Anti-Hel Monoclonal Antibody D1.3 (Vlw92h)

Crystal Structure Of Hen Egg White Lysozyme (Hel) Complexed With The Mutant Anti-Hel Monoclonal Antibody D1.3 (Vlw92f)

Crystal Structure Of Hen Egg White Lysozyme (Hel) Complexed With The Mutant Anti-Hel Monoclonal Antibody D1.3(Vlw92a)

Crystallographic Refinement Of The Three-Dimensional Structure Of The Fab D1.3-Lysozyme Complex At 2.5- Angstroms Resolution

Crystal Structure Of Orthorhombic Lysozyme Grown At Ph 6.5 At 88% Relative Humidity

Crystal Structure Of Orthorhombic Lysozyme Grown At Ph 6.5

Crystal Structure Of The Anti-Lysozyme Antibody Hyhel-63 Complexed With Hen Egg White Lysozyme

Structure Of Hen Egg-White Lysozyme In Complex With Mpd

Crystal Structure Of Hew Lysozyme Under Pressure Of Xenon (8 Bar)

Crystal Structure Of Hyhel-10 Fv-Hen Lysozyme Complex

The 1.8 A Structure Of Microgravity Grown Tetragonal Hen Egg White Lysozyme

The 1.8 A Structure Of Microbatch Oil Drop Grown Tetragonal Hen Egg White Lysozyme

The 1.8 A Structure Of Ground Control Grown Tetragonal Hen Egg White Lysozyme

The 1.8 A Structure Of Gel Grown Tetragonal Hen Egg White Lysozyme

Humanized Anti-Lysozyme Fv Complexed With Lysozyme

Low Temperature Middle Resolution Structure Of Hen Egg White Lysozyme From Masc Data

Orthorhombic Lysozyme Crystallized At High Temperature (310k)

Chicken Egg White Lysozyme Crystal Grown In Bromide Solution

The Structure Of The Orthorhombic Form Of Hen Egg-White Lysozyme At 1.5 Angstroms Resolution

The 1.40 A Structure Of Spacehab-01 Hen Egg White Lysozyme

The 1.33 A Structure Of Tetragonal Hen Egg White Lysozyme

Recombinant hen lysozyme with extra N-terminal Met

Two-disulfide variant of hen lysozyme: 2SS[6-127, 30-115]

Three-disulfide variant of hen lysozyme: C76A/C94A