Letter to Editor: 1H, 15N and 13C resonance assignments of the repeated domain
of human beta ig-h3

Backbone 1H, 15N, and 13C resonance assignments of the repeated domain of human beta ig-h3 protein.

betaig-h3 is a transforming growth factor-beta-inducible cell adhesion molecule
that has four characteristic homologous repeated domains. We made recombinant
betaig-h3 proteins, which were highly active in mediating human corneal
epithelial (HCE) cell adhesion and spreading. The 2nd and the 4th repeated
domains were sufficient to mediate HCE cell adhesion. A sequence analysis
showed that aspartic acid (Asp) and isoleucine (Ile) of the 2nd and the 4th
domains are highly conserved in many fasciclin 1 homologous (fas-1) domains.
Substitution mutational study identified these two amino acids are essential
for cell adhesion. Synthetic peptides containing Asp and Ile, NKDIL and EPDIM
derived from the 2nd and the 4th domains, respectively, almost completely
blocked cell adhesion mediated by not only wild type betaig-h3 but also each of
the 2nd and the 4th domains. These peptides alone were fully active in
mediating cell adhesion. In addition, we demonstrated the functional receptor
for betaig-h3 is alpha(3)beta(1) integrin. These results, therefore, establish
the essential motifs within the 2nd and the 4th domains of betaig-h3, which
interact with alpha(3)beta(1) integrin to mediate HCE cell adhesion to
betaig-h3 and suggest that other proteins containing Asp-Ile in their fas-1
domains could possibly function as cell adhesion molecules.

Kim JE, Kim SJ, Lee BH, Park RW, Kim KS, Kim IS,
Identification of motifs for cell adhesion within the repeated domains of
transforming growth factor-beta-induced gene, betaig-h3
J Biol Chem. 2000 Oct 6;275(40):30907-15

Identification of motifs for cell adhesion within the repeated domains of transforming growth factor-beta-induced gene, betaig-h3.

The molecule has 6 and 7 extra vector-derived amino acids at the N- and
C-terminus respectively. These are excluded from resonance assignments.

Solution Structure Of The Fas1 Domain Of Human Transforming Growth Factor-Beta Induced Protein Ig-H3

The Fourth Fas1 Domain Structure Of Human Bigh3