1H, 13C, and 15N backbone assignment of the vascular endothelial growth factor
receptor-binding domain

vascular endothelial growth factor receptor-binding domain dimer

1H, 13C, and 15N resonance assignment of the vascular endothelial growth factor receptor-binding domain in complex with a receptor-blocking peptide.

Nearly complete sequence-specific 1H, 13C, and 15N resonance assignments are
reported for the backbone atoms of the receptor-binding domain of vascular
endothelial growth factor (VEGF), a 23-kDa homodimeric protein that is a major
regulator of both normal and pathological angiogenesis. The assignment strategy
relied on the use of seven 3D triple-resonance experiments [HN(CO)CA, HNCA,
HNCO, (HCA)CONH, HN(COCA)HA, HN(CA)HA, and CBCA-(CO)NH] and a 3D 15N-TOCSY-HSQC
experiment recorded on a 0.5 mM (12 mg/mL) sample at 500 MHz, pH 7.0, 45
degrees C. Under these conditions, 15N relaxation data show that the protein
has a rotational correlation time of 15.0 ns. Despite this unusually long
correlation time, assignments were obtained for 94 of the 99 residues; 8
residues lack amide 1H and 15N assignments, presumably due to rapid exchange of
the amide 1H with solvent under the experimental conditions used. The secondary
structure of the protein was deduced from the chemical shift indices of the 1H
alpha, 13C alpha, 13C beta, and 13CO nuclei, and from analysis of backbone NOEs
observed in a 3D 15N-NOESY-HSQC spectrum. Two helices and a significant amount
of beta-sheet structure were identified, in general agreement with the
secondary structure found in a recently determined crystal structure of a
similar VEGF construct [Muller YA et al., 1997, Proc Natl Acad Sci USA
94:7192-7197].

Fairbrother WJ, Champe MA, Christinger HW, Keyt BA, Starovasnik MA.
1H, 13C, and 15N backbone assignment and secondary structure of the
receptor-binding domain of vascular endothelial growth factor.
Protein Sci. 1997 Oct;6(10):2250-60.

Protein science : a publication of the Protein Society
1H, 13C, and 15N backbone assignment and secondary structure of the receptor-binding domain of vascular endothelial growth factor.

vascular permeability factor precursor [Homo sapiens]

Vascular Endothelial Growth Factor In Complex With A Neutralizing Antibody

Vascular Endothelial Growth Factor In Complex With An Affinity Matured Antibody

Vegf In Complex With Domain 2 Of The Flt-1 Receptor

Solution Structure Of A Phage-Derived Peptide Antagonist In Complex With Vascular Endothelial Growth Factor

Disulfide Deficient Mutant Of Vascular Endothelial Growth Factor A (C51a And C60a)

Disulfide Deficient Mutant Of Vascular Endothelial Growth Factor A (C57a And C102a)

Disulfide Deficient Mutant Of Vascular Endothelial Growth Factor A (C61a And C104a)

Vascular Endothelial Growth Factor In Complex With Domain 2 Of The Flt-1 Receptor

Crystal Structure Of The Fab Yads1 Complexed With H-Vegf

Crystal Structure Of The Fab Yads2 Complexed With H-Vegf

Structure Of Human Vascular Endothelial Growth Factor

Complex Between Vegf And A Receptor Blocking Peptide

Structure Of The G6 Fab, A Phage Derived Fab Fragment, In Complex With Vegf

Structure Of The B20-4 Fab, A Phage Derived Fab Fragment, In Complex With Vegf

Structure Of Vegf Complexed To A Fab Containing Tyr And Ser In The Cdrs

Vascular Endothelial Growth Factor Refined To 1.93 Angstroms Resolution

Dual Specific Bh1 Fab In Complex With Vegf

Crystal Structure Of An Engineered Human Autonomous Vh Domain In Complex With Vegf

The Crystal Structure Of Chemically Synthesized Vegf-A

The Development Of Peptide-Based Tools For The Analysis Of Angiogenesis

Crystal Structure Of Chemically Synthesized Heterochiral {d-protein Antagonist Plus Vegf-a} Protein Complex In Space Group P21/

Crystal Structure Of Chemically Synthesized Heterochiral {d-protein Antagonist Plus Vegf-a} Protein Complex In Space Group P21

Crystal Structure Of Human Vegf-a Receptor Binding Domain

vascular endothelial growth factor isoform VEGF165 [Homo sapiens]

vascular endothelial growth factor [Felis catus]

vascular endothelial growth factor variant [Homo sapiens]

vascular endothelial growth factor [Homo sapiens]

vascular endothelial growth factor 188 [Canis lupus familiaris]

vascular endothelial growth factor [Callithrix jacchus]

vascular endothelial growth factor A isoform 1 precursor [Canis lupus familiaris]

vascular endothelial growth factor A precursor [Felis catus]

vascular endothelial growth factor A isoform a [Homo sapiens]

vascular endothelial growth factor A isoform c [Homo sapiens]

vascular endothelial growth factor A isoform d [Homo sapiens]

RecName: Full=Vascular endothelial growth factor A; Short=VEGF-A; AltName: Full=Vascular permeability factor; Short=VPF; Flags:

Assignments for vascular endothelial growth factor in complex with v107

Assignments for v107 in complex with vascular endothelial growth factor