Amide NH assignments of the E15C mutant of the armadillo designed protein A4M4A tagged with the diamagnetic PCS tag Lu-4R4S-DOTA-M7 and their corresponding PCSs upon tagging with the paramagnetic tag Tm-4R4S_DOTA-M7

This molecular assembly contains the protein (A4M4A) tagged with
the dia- (Lu-) or paramagnetic (Tm) version of the PCS-trigger
tag (4R4S-DOTA-M7) without the peptide ligand (KR4).

Pseudocontact shifts (PCSs) triggered by the paramagnetic tag Tm-4R4S_DOTA-M7 coupled to the introduced Cys in the sample without ligand.

2 mM TMSP was used to directly reference the proton dimension (0 ppm) and indirectly reference the nitrogen dimension.

Improved Repeat Protein Stability by Combined Consensus and Computational Protein Design.

A 2D 1H-15N HSQC was recorded for each sample. 3D 1H-15N NOESY were recorded only for diamagnetic samples.