N-terminal domain of human HSP90 alpha1 form

Assigned chemical shifts of Carbon methyls were corrected for the deuterium isotop effects. Venters et al. J. Mol. Biol. (1996) 264, 1101-1116.
Assigned chemical shifts Ca, Cb and C' were corrected for the deuterium isotop effects. Venters et al. J. Mol. Biol. (1996) 264, 1101-1116.
Assigned chemical shifts were corrected from the TROSY effects at 600 MHz.

Chemical shifts assignment of human N-HSP90 alpha1 form

Optimized precursor to simplify assignment transfer between backbone resonances and stereospecifically labelled valine and leucine methyl groups: application to human Hsp90 N-terminal domain.

Institut de Biologie Structurale CEA-CNRS-UGA

equipped with 5 mm cryogenically cooled pulsed-field-gradient triple-resonance probe

equipped with a 1.7 mm cryogenically cooled pulsed-field-gradient triple-resonance probe

HSP 90 N-terminal domain, U-[2H, 15N, 13C] 0.5mM, 20 mM Hepes, 150 mM NaCl, 1mM TCEP.

HSP 90 N-terminal domain, U-[2H, 15N, 12C], Ala-[13C1H3]b, Met-[13C1H3]e, Leu/Val-[13C1H3]proS, Ile-[13C1H3]d1, Thr-[13C1H3]g 0.5mM,
20 mM Hepes, 150 mM NaCl, 1mM TCEP.

HSP 90 N-terminal domain, U-[2H, 15N, 12C]-Ala-[13C1H3]b; 20 mM Hepes, 150 mM NaCl, 1mM TCEP.

HSP 90 N-terminal domain, U-[2H, 15N, 12C]-Met-[13C1H3]e; 20 mM Hepes, 150 mM NaCl, 1mM TCEP.

HSP 90 N-terminal domain, U-[2H, 15N, 12C]-Ile-[13C1H3]d1; 20 mM Hepes, 150 mM NaCl, 1mM TCEP.

HSP 90 N-terminal domain, U-[2H, 15N, 12C]-Thr-[13C1H3]g; 20 mM Hepes, 150 mM NaCl, 1mM TCEP.

HSP 90 N-terminal domain, U-[2H, 15N, 12C]-Leu/Val-[13C1H3]proS; 20 mM Hepes, 150 mM NaCl, 1mM TCEP.

NMRPipe: a multidimensional spectral processing system based on UNIX pipes
Frank Delaglio, Stephan Grzesiek, Geerten. W. Vuister, Guang Zhu, John. Pfeifer, and Ad Bax, J. Biomol. NMR. 6, 277-293 (1995)

The CCPN data model for NMR spectroscopy: Development of a software pipeline.
Vranken WF, Boucher W, Stevens TJ, et al. Proteins Struct Funct Genet 59:687-696. (2005)

Monneau et al. Automatic methyl assignment in large proteins by the MAGIC algorithm.
Journal of Biomolecular NMR volume 69, pages 215-227(2017).