NMR assignment of Fc fragment of mouse immunoglobulin G2b glycoprotein galactosylated (G2) glycoform

Fc fragment of mouse immunoglobulin G2b glycoprotein galactosylated (G2) glycoform

alpha-L-fucose; 6-deoxy-alpha-L-galactopyranose; L-fucose; fucose

NMR assignments of the N-glycans of the Fc fragment of mouse immunoglobulin G2b glycoprotein.

The residues of the following glycan are defined as GN1 GN2 M3 M4 GN5 G6 M4' GN5' G6' F:
beta-D-galactopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-2)-alpha-D-mannopyranose-(1-6)-[beta-D-galactopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-2)-alpha-D-mannopyranose-(1-3)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[beta-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose

The structure is illustrated in the manuscript

Fc fragment of mouse immunoglobulin G2b glycoprotein galactosylated, 1

Fc fragment of mouse immunoglobulin G2b glycoprotein galactosylated, 2

Fc fragment of mouse immunoglobulin G2b glycoprotein non-galactosylated (G0) glycoform

Fc fragment of mouse immunoglobulin G2b glycoprotein non-galactosylated

5 mM sodium phosphate buffer (pH 6.0) containing 50 mM NaCl and 10% (v/v) D2O