Proton and nitrogen chemical shift assignments for the chitin-binding domain of
Bacillus circulans WL-12 Chitinase A1

A Chain A, Solution Structure Of The Chitin-Binding Domain Of Bacillus Circulans Wl-12 Chitinase A1

Solution structure of the chitin-binding domain of Bacillus circulans WL-12 chitinase A1.

The protein has a T7 tag consisting of 14 residues (H2N-Met-Ala-Ser-Met-Thr-Gly-Gly-Gly-Gly-Met-Gly-Arg-Gly-Ser-) derived from the expression vector at its N-terminus.
The calculated weight of the molecule, without the T7 tag region, is 5,018.

intein-CBD, partial [synthetic construct]

DnaB-Ano fusion protein [synthetic construct]

GyrA-Ano fusion protein [synthetic construct]

RIR1-Ano fusion protein [synthetic construct]

chitin binding domain [Expression vector pNam]

RNA polymerase beta prime subunit/intein-CBD fusion protein [Cloning vector pIA423]

SspDnaE-C/CBD fusion protein [Expression vector pSFBAD09]

glutaryl 7-aminocephalosporanic acid acylase/modified chitin binding domain fusion protein [synthetic construct]

RecName: Full=Chitinase A1; Flags: Precursor

Samples adjusted to pH 6.0 in H2O was lyophilized and then dissolved in the same amount of D2O.