Solution NMR structure of NF7; de novo designed protein with a novel fold

Chemical shift reference of 1H was based on the proton of water (4.784ppm at 298K) and then those of 15N and 13C were calculated based on their gyro-magnetic ratios.

Exploration of novel αβ-protein folds through de novo design.

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Solution NMR structure of NF1; de novo designed protein with a novel fold

Solution NMR structure of NF2; de novo designed protein with a novel fold

Solution NMR structure of NF5; de novo designed protein with a novel fold

Solution NMR structure of NF6; de novo designed protein with a novel fold

Solution NMR structure of NF4; de novo designed protein with a novel fold

Solution NMR structure of NF8 (knot fold); de novo designed protein with a novel fold

Solution NMR structure of NF3; de novo designed protein with a novel fold

0.9 mM [U-100% 13C; U-100% 15N] NF7, 4.3 mM sodium phosphate, 5.7 mM potassium phosphate, 50 mM sodium chloride, 95% H2O/5% D2O

0.2 mM [U-100% 13C; U-100% 15N] NF7, 4.3 mM sodium phosphate, 5.7 mM potassium phosphate, 50 mM sodium chloride, 4.7 mg/mL Pf1 phage, 95% H2O/5% D2O

Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo, ... and Kollman

Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax