SOLUTION STRUCTURE OF LYS39 ACETYLATED HUMAN SUMO1

Tolerance values used in automated NOESY assignments.

Lys39 acetylated mature SMALL UBIQUITIN-RELATED MODIFIER 1 (SUMO1)

Residues 1-14 (MGSSHHHHHHSQDP) represent a non-native purification tag. These residues were neither assigned nor included in structure calculation.

PLASMID PCDF PYLT-1 WITH SUMO INSERT WITH K39STOP MUTATION (WITH AMBER CODON) AND PACKRS-3 AS DESCRIBED IN NEUMANN ET AL., MOL CELL, 36, 153, 2009

NMR DATA WAS ACQUIRED AT 295K USING SHIGEMI NMR TUBES.

TORSION ANGLE DYNAMICS, DGSA- DISTANCE GEOMETRY SIMULATED ANNEALING

SOLUTION STRUCTURE OF LYS37 ACETYLATED HUMAN SUMO1

SOLUTION STRUCTURE OF LYS42 ACETYLATED HUMAN SUMO2

SOLUTION STRUCTURE OF LYS33 ACETYLATED HUMAN SUMO2

1 mM [U-100% 13C; U-100% 15N] SUMO1 K39Ac, 10 mM potassium phosphate, 100 mM potassium chloride, 2 mM DTT, 0.1 mM EDTA, 0.001 % sodium azide, 90% H2O/10% D2O

1 mM [U-100% 15N] SUMO1 K39Ac, 10 mM potassium phosphate, 100 mM potassium chloride, 2 mM DTT, 0.1 mM EDTA, 0.001 % sodium azide, 90% H2O/10% D2O

1 mM [U-100% 15N] SUMO1 K39Ac, 10 mM potassium phosphate, 100 mM potassium chloride, 2 mM DTT, 0.1 mM EDTA, 0.001 % sodium azide, 10 mg/mL Pf1 phage, 90% H2O/10% D2O

1 mM SUMO1 K39Ac, 10 mM potassium phosphate, 100 mM potassium chloride, 2 mM DTT, 0.1 mM EDTA, 0.001 % sodium azide, 90% H2O/10% D2O

Schwieters, Kuszewski, Tjandra and Clore