NMR solution structure of the heavy metal binding domain of P1B-ATPase LpCopA.

residues 1-35 and 71-83 are disordered and, hence, are not modeled in the coordinate file

100 mg/mL [U-100% 13C; U-100% 15N] Heavy metal binding domain (HMBD) of LpCopA,
50 mM 2-(N-morpholino)ethanesulfonic acid, 100 mM KCl,
2 mM TCEP (tris(2-carboxyethyl)phosphine), 90% H2O/10% D2O

100 mg/mL [U-100% 13C; U-100% 15N] Heavy metal binding domain (HMBD) of LpCopA,
13 mM AgNO3, 50 mM 2-(N-morpholino)ethanesulfonic acid, 100 mM KCl,
2 mM TCEP (tris(2-carboxyethyl)phosphine), 90% H2O/10% D2O

Heavy metal binding domain (HMBD) of LpCopA

Schwieters, Kuszewski, Tjandra and Clore