Solution structure and 1H, 13C and 15N chemical shift assignments for the complex of NECAP1 PHear domain with phosphorylated AP2 mu2 148-163

Adaptin ear-binding coat-associated protein 1, AP-2 complex subunit mu

(2S,3R)-2-amino-3-phosphonooxy-butanoic acid

2D 1H-1H NOESY (reject 15N,13C coupled 1H in F1, accept 15N,13C coupled 1H in F2) tau(m) 150ms

2D 1H-1H NOESY (reject 15N,13C coupled 1H in F1 and F2) tau(m) 150ms

2D 1H-1H TOCSY (reject 15N,13C coupled 1H in F2)

2D 1H-13C HSQC aliphatic (constant time)

2D 1H-1H NOESY (reject 15N,13C coupled 1H in F1, accept 13C coupled 1H in F2) tau(m) 150ms

3D 1H-13C NOESY aliphatic (reject 15N,13C coupled 1H in F1) tau(m) 150ms

3D 1H-13C NOESY aromatic (reject 15N,13C coupled 1H in F1) tau(m) 150ms

separate tube (no insert) similar to the experimental sample tube

Temporal Ordering in Endocytic Clathrin-Coated Vesicle Formation via AP2 Phosphorylation.

Adaptor-related protein complex 2 subunit mu

Clathrin assembly protein complex 2 mu medium chain

Plasma membrane adaptor AP-2 50 kDa protein

torsion angle dynamicssimulated annealingmolecular dynamics
Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo, ... and Kollman
Schwieters, Kuszewski, Tjandra and Clore